General Stability | Organism |
---|---|
the enzyme is stable under NaCl concentrations between 1.8 and 4.8 M in the absence or presence of chloroform | Haloarcula sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | - |
Haloarcula sp. | |
Cu2+ | 20 mM | Haloarcula sp. | |
Cyclohexane | inhibitory effect of cyclooctane on amylase stability is stronger than that of cyclohexane | Haloarcula sp. | |
cyclooctane | inhibitory effect of cyclooctane on amylase stability is stronger than that of cyclohexane | Haloarcula sp. | |
EDTA | 515 mM, with or without chloroform | Haloarcula sp. | |
ethanol | - |
Haloarcula sp. | |
Fe2+ | 20 mM | Haloarcula sp. | |
Fe3+ | 20 mM | Haloarcula sp. | |
additional information | not inhibited by aromatic hydrocarbon (benzene, etc.) | Haloarcula sp. | |
Ni2+ | 20 mM | Haloarcula sp. | |
Rb+ | 20 mM | Haloarcula sp. | |
Zn2+ | 20 mM | Haloarcula sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Haloarcula sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | optimal NaCl concentration in the absence of chloroform is 4.3 M. No activity is detected below 1.7 M NaCl. In the presence of chloroform, optimal NaCl concentration is 4.3 M, and activity is not detected below 0.9 M | Haloarcula sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
gel filtration | Haloarcula sp. |
70000 | - |
1 * 70000, SDS-PAGE | Haloarcula sp. |
Organic Solvent | Comment | Organism |
---|---|---|
benzene | stable in presence of | Haloarcula sp. |
chloroform | stable in presence of | Haloarcula sp. |
n-decane | stable in presence of | Haloarcula sp. |
n-nonane | stable in presence of | Haloarcula sp. |
octane | stable in presence of | Haloarcula sp. |
styrene | stable in presence of | Haloarcula sp. |
toluene | stable in presence of | Haloarcula sp. |
xylene | stable in presence of | Haloarcula sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula sp. | - |
- |
- |
Haloarcula sp. S-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloarcula sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.07 mg of starch/min*mg | Haloarcula sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Haloarcula sp. | maltose + maltotriose + D-glucose | major reaction products of the amylase are maltose and maltotriose, and the minor product is glucose | ? | |
starch + H2O | - |
Haloarcula sp. S-1 | maltose + maltotriose + D-glucose | major reaction products of the amylase are maltose and maltotriose, and the minor product is glucose | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70000, SDS-PAGE | Haloarcula sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
in buffer containing 4.3 M NaCl, pH 7.0 | Haloarcula sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 7.0, 50 mM Tris-HCl buffer containing 4.3 M NaCl, 30 min, no loss of activity | Haloarcula sp. |
70 | - |
pH 7.0, 50 mM Tris-HCl buffer containing 4.3 M NaCl, 30 min, 50% loss of activity | Haloarcula sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Haloarcula sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 25°C, 30 min, stable | Haloarcula sp. |