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Literature summary for 3.2.1.1 extracted from

  • Perez-Pomares, F.; Bautista, V.; Ferrer, J.; Pire, C.; Marhuenda-Egea, F.C.; Bonete, M.J.
    alpha-Amylase activity from the halophilic archaeon Haloferax mediterranei (2003), Extremophiles, 7, 299-306.
    View publication on PubMed
Search for more literature for 3.2.1.1

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ slight activator at concentrations below 150 mM, activating only a maximum of 3% at 100 mM, and a slight inhibitor at higher concentrations (8% of the initial activity at 200 mM) Haloferax mediterranei

General Stability

General Stability Organism
the enzyme is stable from 2 to 4 M NaCl Haloferax mediterranei

Inhibitors

Inhibitors Comment Organism Structure
EDTA pH 7.5, irreversible loss of activity, half-life: 29 h at 25 mM, 64 h at 12 mM, 670 h at 6 mM and 3500 at 3 mM Haloferax mediterranei

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular when this medium is enriched with starch, alpha-amylase activity is excreted to the medium in low concentration Haloferax mediterranei
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activator at concentrations below 150 mM, activating only a maximum of 3% at 100 mM, and a slight inhibitor at higher concentrations (8% of the initial activity at 200 mM) Haloferax mediterranei
Mg2+ inhibitor when added in concentrations from 50 mM to 200 mM. The maximum inhibitory effect is obtained at 100 mM, inhibiting only 10% of the initial activity Haloferax mediterranei
NaCl maximal activity at 3 M NaCl Haloferax mediterranei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Haloferax mediterranei
58000
-
 1 * 58000, SDS-PAGE Haloferax mediterranei

Organism

Organism UniProt Comment Textmining
Haloferax mediterranei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloferax mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclodextrin + H2O the activity with alpha-cyclodextrin is 46% of the activity with starch in the same conditions at 0.5% (w/v) substrate concentrations Haloferax mediterranei ?
-
 ?
beta-cyclodextrin + H2O the activity with alpha-cyclodextrin is 48% of the activity with starch in the same conditions at 0.5% (w/v) substrate Haloferax mediterranei ?
-
 ?
starch + H2O endo-acting activity Haloferax mediterranei ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 58000, SDS-PAGE Haloferax mediterranei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
 Haloferax mediterranei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 50-60: temperature optimum, 80°C: 65% of maximal activity Haloferax mediterranei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 7.0, half-life: 2100 h Haloferax mediterranei
50
-
 pH 7.0, half-life: 10.1 h Haloferax mediterranei
60
-
 pH 7.0, half-life: 0.88 h Haloferax mediterranei
70
-
 pH 7.0, half-life: 7.2 min Haloferax mediterranei
80
-
 pH 7.0, half-life: 4.1 min Haloferax mediterranei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Haloferax mediterranei

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.2
-
 at 3 M NaCl, half-life: 5.7 h Haloferax mediterranei
4.6
-
 at 3 M NaCl, half-life: 138 h Haloferax mediterranei
5
-
 at 3 M NaCl, half-life: 15000 h Haloferax mediterranei
6 8 at 3 M NaCl, half-life: 100 d Haloferax mediterranei
9
-
 at 3 M NaCl, half-life: 280 d Haloferax mediterranei
10
-
 at 3 M NaCl, half-life: 240 d Haloferax mediterranei