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Literature summary for 3.2.1.1 extracted from

  • Leveque, E.; Haye, B.; Belarbi, A.
    Cloning and expression of an alpha-amylase encoding gene from the hyperthermophilic archaebacterium Thermococcus hydrothermalis and biochemical characterisation of the recombinant enzyme (2000), FEMS Microbiol. Lett., 186, 67-71.
    View publication on PubMed
Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherchia coli Thermococcus hydrothermalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51544
-
 x * 51544, calculated from sequence Thermococcus hydrothermalis
53600
-
 x * 53600, SDS-PAGE Thermococcus hydrothermalis

Organism

Organism UniProt Comment Textmining
Thermococcus hydrothermalis O93647
-
-
Thermococcus hydrothermalis AL662 O93647
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus hydrothermalis

Subunits

Subunits Comment Organism
? x * 51544, calculated from sequence Thermococcus hydrothermalis
? x * 53600, SDS-PAGE Thermococcus hydrothermalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75 85
-
 Thermococcus hydrothermalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 110 50°C: about 45% of maximal activity, 110°C: about 40% of maximal activity Thermococcus hydrothermalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
 Thermococcus hydrothermalis

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 pH 4.5: about 75% of maximal activity, pH 7.0: about 55% of maximal activity Thermococcus hydrothermalis