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Literature summary for 3.2.1.1 extracted from

  • Yadav, J.K.; Prakash, V.
    Thermal stability of alpha-amylase in aqueous cosolvent systems (2009), J. Biosci., 34, 377-387.
    View publication on PubMed

General Stability

General Stability Organism
more than 90% of enzyme activity is preserved even after 40 min of incubation at 60°C in the presence of 40% (w/v) glycerol Bacillus sp. (in: Bacteria)
more than 90% of enzyme activity is preserved even after 40 min of incubation at 60°C in the presence of 40% (w/v) sorbitol Bacillus sp. (in: Bacteria)
more than 90% of enzyme activity is preserved even after 40 min of incubation at 60°C in the presence of 40% (w/v) sucrose Bacillus sp. (in: Bacteria)
more than 90% of enzyme activity is preserved even after 40 min of incubation at 60°C in the presence of 40% (w/v) trehalose, in the presence of 20% (w/v) trehalose 45% activity is retained even at 80°C Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-chloro-4-nitrophenyl alpha-D-maltotrioside + H2O Bacillus sp. (in: Bacteria)
-
2-chloro-4-nitrophenol + alpha-D-maltotriose
-
?
starch + H2O Bacillus sp. (in: Bacteria)
-
maltose + ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-4-nitrophenyl alpha-D-maltotrioside + H2O
-
Bacillus sp. (in: Bacteria) 2-chloro-4-nitrophenol + alpha-D-maltotriose
-
?
starch + H2O
-
Bacillus sp. (in: Bacteria) maltose + ?
-
?

Synonyms

Synonyms Comment Organism
alpha-amylase type II
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 60 increasing the temperature above 55°C leads to irreversible thermal inactivation of the enzyme, there is complete loss of alpha-amylase activity after 30 min of incubation at 60°C Bacillus sp. (in: Bacteria)