Cloned (Comment) | Organism |
---|---|
expressed in Bacillus subtilis strain WB600 | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + H2O | Bacillus subtilis | - |
malto-oligosaccharides | - |
? | |
soluble starch + H2O | Bacillus subtilis BF768 | - |
malto-oligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | B8Y1H0 | - |
- |
Bacillus subtilis BF768 | B8Y1H0 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-75gel filtration | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the recombinant enzyme shows a specific activity of 21.3 units/mg in the extract supernatant and of 35.8 units/mg after 1.7fold purification, one unit is defined as the amount of enzyme required to liquefy 1 g soluble starch completely for 1 h at 60°C and pH 6.0 | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + H2O | - |
Bacillus subtilis | malto-oligosaccharides | - |
? | |
soluble starch + H2O | - |
Bacillus subtilis BF768 | malto-oligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,4-glucan-4-glucanohydrolase | - |
Bacillus subtilis |
AmyE | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant enzyme | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | after incubation at 40, 60 and 80°C for 1 h, the recombinant enzyme retains nearly 90, 80 and 50% of its initial activity, respectively. 83% of its initial activity is lost after being treated at 100°C for 20 min | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
purified recombinant enzyme | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | - |
Bacillus subtilis |