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Literature summary for 3.2.1.1 extracted from
- Hyperthermostable, Ca(2+)-independent, and high maltose-forming alpha-amylase production by an extreme thermophile Geobacillus thermoleovorans: Whole cell immobilization (2009), Appl. Biochem. Biotechnol., 159, 464-477.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | alpha-amylase production, the addition of cAMP to the medium containing glucose does not result in the repression of enzyme synthesis. The addition of 1% maltose to the starch arginine medium results in a twofold enhancement in enzyme titers. Polyurethane foam-immobilzed cells secret alpha-amylase, which is higher than that with the free cells | Geobacillus thermoleovorans | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Ca2+-independent | Geobacillus thermoleovorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermoleovorans | - |
NP 54, isolated from a water sample collected from the hot water spring of the Waimangu volcanic valley in New Zealand | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | alpha-amylase production, the addition of cAMP to the medium containing glucose does not result in the repression of enzyme synthesis. The addition of 1% maltose to the starch arginine medium results in a twofold enhancement in enzyme titers. Polyurethane foam-immobilzed cells secret alpha-amylase, which is higher than that with the free cells | Geobacillus thermoleovorans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
28 U/ml, pH 8.0, 100°C | Geobacillus thermoleovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | pH 8.0, 100°C | Geobacillus thermoleovorans | maltose + ? | - |
? |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
assay at | Geobacillus thermoleovorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Geobacillus thermoleovorans |
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