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Literature summary for 3.2.1.1 extracted from

  • Pelegrini, P.B.; Lay, F.T.; Murad, A.M.; Anderson, M.A.; Franco, O.L.
    Novel insights on the mechanism of action of alpha-amylase inhibitors from the plant defensin family (2008), Proteins, 73, 719-729.
    View publication on PubMed
Search for more literature for 3.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
additional information Vigna unguiculata defensin i.e. VuD1, plant defensins are small protein consisting of 45-54 amino acids, no inhibition with 0.1 mg/ml inhibitor, pH 6.5, 37°C Aspergillus fumigatus
Vigna unguiculata defensin i.e. VuD1, plant defensins are small protein consisting of 45-54 amino acids, below 62% inhibition with 0.1 mg/ml inhibitor, pH 6.5, 37°C Acanthoscelides obtectus
Vigna unguiculata defensin i.e. VuD1, plant defensins are small protein consisting of 45-54 amino acids, below 5% inhibition with 0.1 mg/ml inhibitor, pH 6.5, 37°C Callosobruchus maculatus
Vigna unguiculata defensin i.e. VuD1, plant defensins are small protein consisting of 45-54 amino acids, ca. 38% inhibition with 0.1 mg/ml inhibitor, pH 6.5, 37°C Homo sapiens
Vigna unguiculata defensin i.e. VuD1, plant defensins are small protein consisting of 45-54 amino acids, ca. 12% inhibition with 0.1 mg/ml inhibitor, pH 6.5, 37°C, low inhibition activity in pig is probably related to extended loops in the structural core of the amylase, reducing the contact between defensin VuD1 and the catalytic site Sus scrofa
Vigna unguiculata defensin i.e. VuD1, plant defensins are small protein consisting of 45-54 amino acids, below 60% inhibition with 0.1 mg/ml inhibitor, pH 6.5, 37°C, Lys1 from the defensin VuD1 appears to interact with residue Asp204 from the anylase ZSA, which is located inside the catalytic site, analyzed by in silico docking experiments Zabrotes subfasciatus

Organism

Organism UniProt Comment Textmining
Acanthoscelides obtectus
-
 bean weevil
-
Aspergillus fumigatus
-
-
-
Callosobruchus maculatus
-
-
-
Homo sapiens
-
-
-
Sus scrofa P00690
-
-
Zabrotes subfasciatus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
 Zabrotes subfasciatus
-
larva
-
 Acanthoscelides obtectus
-
larva
-
 Callosobruchus maculatus
-
midgut
-
 Zabrotes subfasciatus
-
midgut
-
 Acanthoscelides obtectus
-
midgut
-
 Callosobruchus maculatus
-
pancreas
-
 Sus scrofa
-
saliva
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
 Homo sapiens ?
-
 ?
starch + H2O
-
 Aspergillus fumigatus ?
-
 ?
starch + H2O pH 6.5, 37°C Zabrotes subfasciatus ?
-
 ?
starch + H2O pH 6.5, 37°C Acanthoscelides obtectus ?
-
 ?
starch + H2O pH 6.5, 37°C Callosobruchus maculatus ?
-
 ?
starch + H2O pH 6.5, 37°C Sus scrofa ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-amylase Aasp
-
 Aspergillus fumigatus
alpha-amylase AOA
-
 Acanthoscelides obtectus
alpha-amylase CMA
-
 Callosobruchus maculatus
alpha-amylase HA
-
 Homo sapiens
alpha-amylase PPA
-
 Sus scrofa
alpha-amylase ZSA
-
 Zabrotes subfasciatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Aspergillus fumigatus
37
-
 assay at Zabrotes subfasciatus
37
-
 assay at Acanthoscelides obtectus
37
-
 assay at Callosobruchus maculatus
37
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Homo sapiens
6.5
-
 assay at Aspergillus fumigatus
6.5
-
 assay at Zabrotes subfasciatus
6.5
-
 assay at Acanthoscelides obtectus
6.5
-
 assay at Callosobruchus maculatus
6.5
-
 assay at Sus scrofa