Application | Comment | Organism |
---|---|---|
nutrition | potentially useful in bread making as antistaling agent, economic production using low-cost sugarcane bagasse possible | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag2+ | activity severely inhibited, indicates the role of sulfydryl group in catalysis | Bacillus subtilis | |
EDTA | 50% of the activity abolished in presence of 50 mM EDTA | Bacillus subtilis | |
Hg2+ | activity severely inhibited, indicates the role of sulfydryl group in catalysis | Bacillus subtilis | |
p-hydroxymercurybenzoate | activity severely inhibited, indicates the role of sulfydryl group in catalysis | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reaction products determined by HPLC, kinetic properties of native and purified protein shown | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag2+ | activity inhibited | Bacillus subtilis | |
Ba2+ | measured activity about 8090% | Bacillus subtilis | |
Ca2+ | activity not significantly enhanced in presence of 1 mM CaCl2 | Bacillus subtilis | |
Cu2+ | measured activity about 8090% | Bacillus subtilis | |
Fe3+ | activity about 120% | Bacillus subtilis | |
Hg2+ | activity inhibited | Bacillus subtilis | |
Mn2+ | measured activity about 8090% | Bacillus subtilis | |
Ni2+ | measured activity about 8090% | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
gel filtration, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Bacillus subtilis | synthesis of alpha-amylase not catabolite-repressed by glucose in strain KCC103, difference to other Bacillus species | maltooligosaccharides | - |
? | |
starch + H2O | Bacillus subtilis KCC103 | synthesis of alpha-amylase not catabolite-repressed by glucose in strain KCC103, difference to other Bacillus species | maltooligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | A8VWC5 | amyE | - |
Bacillus subtilis KCC103 | A8VWC5 | amyE | - |
Purification (Comment) | Organism |
---|---|
from crude cell extracts using acetone precipitation and gel filtration, yields of 45% obtained | Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | activity tested, purified from | Bacillus subtilis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
kinetic properties determined by amount of reducing sugars, assayed according to the dinitrosalicylic acid method | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | synthesis of alpha-amylase not catabolite-repressed by glucose in strain KCC103, difference to other Bacillus species | Bacillus subtilis | maltooligosaccharides | - |
? | |
starch + H2O | endolytic activity by forming maltooligosaccharides on hydrolysis of soluble starch, similar product profiles at pH 4 and pH 7, rather small maltooligosaccharides (D2D4) than larger maltooligosaccharides (D5D7) formed, proportional levels of sugars produced after 24 h hydrolysis of soluble starch indicated | Bacillus subtilis | maltooligosaccharides | - |
? | |
starch + H2O | synthesis of alpha-amylase not catabolite-repressed by glucose in strain KCC103, difference to other Bacillus species | Bacillus subtilis KCC103 | maltooligosaccharides | - |
? | |
starch + H2O | endolytic activity by forming maltooligosaccharides on hydrolysis of soluble starch, similar product profiles at pH 4 and pH 7, rather small maltooligosaccharides (D2D4) than larger maltooligosaccharides (D5D7) formed, proportional levels of sugars produced after 24 h hydrolysis of soluble starch indicated | Bacillus subtilis KCC103 | maltooligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-amylase | - |
Bacillus subtilis |
AmyCR | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
activity measured between 30°C and 80°C at pH 6, optimal activity ranges between 65 and 70°C, half-life of 7 min at 70°C | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 75 | retention of 80% of the maximum activity at 75°C, rapid deactivation at 70°C with a half-life of 7 min | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
moderate thermostability, half-life of 94 min at 50°C, rapid deactivation with a half-life of 7 min at 70°C, analyzed at pH 6 and temperatures between 30°C to 70°C, residual activity measured in intervals of 10 min | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
optimum of enzyme activity from pH 6 to pH 7 | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | activity determined | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | incubation at different pH conditions from pH 4.5 to pH 9 for 24h in 4°C followed by activity measurement, retention of more than 98% of initial activity | Bacillus subtilis |