Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.1 extracted from

  • Cho, M.H.; Park, S.E.; Lee, M.H.; Ha, S.J.; Kim, H.Y.; Kim, M.J.; Lee, S.J.; Madsen, S.M.; Park, C.S.
    Extracellular secretion of a maltogenic amylase from Lactobacillus gasseri ATCC33323 in Lactococcus lactis MG1363 and its application on the production of branched maltooligosaccharides (2007), J. Microbiol. Biotechnol., 17, 1521-1526.
    View publication on PubMed

Application

Application Comment Organism
nutrition potentially useful in bread making as antistaling agent, economic production using low-cost sugarcane bagasse possible Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ activity severely inhibited, indicates the role of sulfydryl group in catalysis Bacillus subtilis
EDTA 50% of the activity abolished in presence of 50 mM EDTA Bacillus subtilis
Hg2+ activity severely inhibited, indicates the role of sulfydryl group in catalysis Bacillus subtilis
p-hydroxymercurybenzoate activity severely inhibited, indicates the role of sulfydryl group in catalysis Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information reaction products determined by HPLC, kinetic properties of native and purified protein shown Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ag2+ activity inhibited Bacillus subtilis
Ba2+ measured activity about 80–90% Bacillus subtilis
Ca2+ activity not significantly enhanced in presence of 1 mM CaCl2 Bacillus subtilis
Cu2+ measured activity about 80–90% Bacillus subtilis
Fe3+ activity about 120% Bacillus subtilis
Hg2+ activity inhibited Bacillus subtilis
Mn2+ measured activity about 80–90% Bacillus subtilis
Ni2+ measured activity about 80–90% Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
gel filtration, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Bacillus subtilis synthesis of alpha-amylase not catabolite-repressed by glucose in strain KCC103, difference to other Bacillus species maltooligosaccharides
-
?
starch + H2O Bacillus subtilis KCC103 synthesis of alpha-amylase not catabolite-repressed by glucose in strain KCC103, difference to other Bacillus species maltooligosaccharides
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis A8VWC5 amyE
-
Bacillus subtilis KCC103 A8VWC5 amyE
-

Purification (Commentary)

Purification (Comment) Organism
from crude cell extracts using acetone precipitation and gel filtration, yields of 45% obtained Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant activity tested, purified from Bacillus subtilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
kinetic properties determined by amount of reducing sugars, assayed according to the dinitrosalicylic acid method Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O synthesis of alpha-amylase not catabolite-repressed by glucose in strain KCC103, difference to other Bacillus species Bacillus subtilis maltooligosaccharides
-
?
starch + H2O endolytic activity by forming maltooligosaccharides on hydrolysis of soluble starch, similar product profiles at pH 4 and pH 7, rather small maltooligosaccharides (D2–D4) than larger maltooligosaccharides (D5–D7) formed, proportional levels of sugars produced after 24 h hydrolysis of soluble starch indicated Bacillus subtilis maltooligosaccharides
-
?
starch + H2O synthesis of alpha-amylase not catabolite-repressed by glucose in strain KCC103, difference to other Bacillus species Bacillus subtilis KCC103 maltooligosaccharides
-
?
starch + H2O endolytic activity by forming maltooligosaccharides on hydrolysis of soluble starch, similar product profiles at pH 4 and pH 7, rather small maltooligosaccharides (D2–D4) than larger maltooligosaccharides (D5–D7) formed, proportional levels of sugars produced after 24 h hydrolysis of soluble starch indicated Bacillus subtilis KCC103 maltooligosaccharides
-
?

Synonyms

Synonyms Comment Organism
alpha-amylase
-
Bacillus subtilis
AmyCR
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
activity measured between 30°C and 80°C at pH 6, optimal activity ranges between 65 and 70°C, half-life of 7 min at 70°C Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 75 retention of 80% of the maximum activity at 75°C, rapid deactivation at 70°C with a half-life of 7 min Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
moderate thermostability, half-life of 94 min at 50°C, rapid deactivation with a half-life of 7 min at 70°C, analyzed at pH 6 and temperatures between 30°C to 70°C, residual activity measured in intervals of 10 min Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
optimum of enzyme activity from pH 6 to pH 7 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
5 7 activity determined Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 incubation at different pH conditions from pH 4.5 to pH 9 for 24h in 4°C followed by activity measurement, retention of more than 98% of initial activity Bacillus subtilis