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Literature summary for 3.2.1.1 extracted from

  • Champreda, V.; Kanokratana, P.; Sriprang, R.; Tanapongpipat, S.; Eurwilaichitr, L.
    Purification, biochemical characterization, and gene cloning of a new extracellular thermotolerant and glucose tolerant maltooligosaccharide-forming alpha-amylase from an endophytic ascomycete Fusicoccum sp. BCC4124 (2007), Biosci. Biotechnol. Biochem., 71, 2010-2020.
    View publication on PubMed

Application

Application Comment Organism
biotechnology the thermotolerant, glucose tolerant maltooligosaccharide-forming alpha-amylase is potent for biotechnological application Fusicoccum sp.

Cloned(Commentary)

Cloned (Comment) Organism
-
Fusicoccum sp.

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 2 mM, complete inhibition Fusicoccum sp.
glucose 1 M, 34% inhibition. No inhibition up to 0.25 M Fusicoccum sp.
maltose 0.25 M, 69% inhibition Fusicoccum sp.
Mn2+ 2 mM, about 70% inhibition Fusicoccum sp.
Ni2+ 2 mM, about 15% inhibition Fusicoccum sp.
Zn2+ 2 mM, about 85% inhibition Fusicoccum sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-value: 5.2 mg/ml for soluble starch, 14.46 mg/ml for beta-cyclodextrin, 9.18 mg/ml for gamma-cyclodextrin Fusicoccum sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Fusicoccum sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2 mM, about 1.2fold activation Fusicoccum sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, SDS-PAGE Fusicoccum sp.

Organism

Organism UniProt Comment Textmining
Fusicoccum sp.
-
-
-
Fusicoccum sp. BCC4124
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Fusicoccum sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
838.9
-
-
Fusicoccum sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-cyclodextrin + H2O
-
Fusicoccum sp. ?
-
?
beta-cyclodextrin + H2O
-
Fusicoccum sp. BCC4124 ?
-
?
gamma-cyclodextrin + H2O
-
Fusicoccum sp. ?
-
?
gamma-cyclodextrin + H2O
-
Fusicoccum sp. BCC4124 ?
-
?
maltoheptaose + H2O
-
Fusicoccum sp. D-glucose + maltose + maltotriose
-
?
maltohexaose + H2O
-
Fusicoccum sp. D-glucose + maltose + maltotriose
-
?
maltopentaose + H2O
-
Fusicoccum sp. glucose + maltose + maltotriose
-
?
maltotetraose + H2O
-
Fusicoccum sp. glucose + maltose + maltotriose
-
?
maltotriose + H2O
-
Fusicoccum sp. maltose + D-glucose
-
?
maltotriose + H2O
-
Fusicoccum sp. BCC4124 maltose + D-glucose
-
?
additional information trace activity towards alpha-cyclodextrin, no hydrolysis of pullulan, no hydrolysis of maltose Fusicoccum sp. ?
-
?
additional information trace activity towards alpha-cyclodextrin, no hydrolysis of pullulan, no hydrolysis of maltose Fusicoccum sp. BCC4124 ?
-
?
soluble starch + H2O maltose, maltotriose, and maltotetraose are the major products from starch hydrolysis but prolonged reaction leads to the production of glucose, maltose, and maltotriose Fusicoccum sp. ?
-
?
soluble starch + H2O maltose, maltotriose, and maltotetraose are the major products from starch hydrolysis but prolonged reaction leads to the production of glucose, maltose, and maltotriose Fusicoccum sp. BCC4124 ?
-
?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Fusicoccum sp.

Synonyms

Synonyms Comment Organism
Amy-FC1
-
Fusicoccum sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Fusicoccum sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 75 30°C: about 60% of maximal activity, 75°C: about 85% of maximal activity Fusicoccum sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
1 h, less than 5% loss of activity Fusicoccum sp.
60
-
1 h, complete inactivation in absence of substrate, less than 10% inactivation in presence of soluble starch. In presence of Ca2+ and Sr2+, Amy-FC1 retains full activity after 1 h and more than 80% activity after 5 h Fusicoccum sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.025
-
soluble starch
-
Fusicoccum sp.
283.3
-
beta-cyclodextrin
-
Fusicoccum sp.
485
-
beta-cyclodextrin
-
Fusicoccum sp.
561.7
-
soluble starch
-
Fusicoccum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Fusicoccum sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
50°C, 1 h, about 40% loss of activity Fusicoccum sp.
7 8 50°C, 1 h, no loss of activity Fusicoccum sp.
9
-
50°C, 1 h, about 60% loss of activity Fusicoccum sp.