Application | Comment | Organism |
---|---|---|
food industry | the maltooligosaccharide forming endo-alpha-amylase is useful in bread making as an antistaling agent and it can be produced economically using low-cost sugarcane bagasse | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Bacillus subtilis | |
EDTA | 50 mM, abolished 50% of the activity | Bacillus subtilis | |
Hg2+ | - |
Bacillus subtilis | |
p-hydroxymercuribenzoate | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM for starch is 2.6 mg/ml | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis KCC103 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
482.4 | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + H2O | endolytic activity. Small maltooligosaccharides (D2-D4) are formed more predominantly than larger maltooligosaccharides (D5-D7) | Bacillus subtilis | maltooligosaccharides | - |
? | |
soluble starch + H2O | endolytic activity. Small maltooligosaccharides (D2-D4) are formed more predominantly than larger maltooligosaccharides (D5-D7) | Bacillus subtilis KCC103 | maltooligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 70 | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life: 7 min | Bacillus subtilis |
70 | - |
half-life: 94 min | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | active in the range | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | stable | Bacillus subtilis |