Any feedback?
Literature summary for 3.2.1.1 extracted from
- Enzymic properties of an alkaline chelator-resistant alpha-amylase from an alkaliphilic Bacillus sp. isolate L1711 (2005), Process Biochem., 40, 2401-2408.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | activation of isozyme BAA at 5-10 mM | Bacillus sp. (in: Bacteria) |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | strong inhibition of isozyme BAA | Bacillus sp. (in: Bacteria) | |
| Ca2+ | strong inhibition of isozyme BAA | Bacillus sp. (in: Bacteria) | |
| Cu2+ | strong inhibition of isozyme BAA | Bacillus sp. (in: Bacteria) | |
| Mg2+ | strong inhibition of isozyme BAA | Bacillus sp. (in: Bacteria) | |
| Mn2+ | strong inhibition of isozyme BAA | Bacillus sp. (in: Bacteria) | |
| PMSF | - |
Bacillus sp. (in: Bacteria) | |
| Zn2+ | strong inhibition of isozyme BAA | Bacillus sp. (in: Bacteria) | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
soluble starch | pH 10.0, 40°C | Bacillus sp. (in: Bacteria) |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activation of isozyme BAA at 10 mM | Bacillus sp. (in: Bacteria) | |
| Na+ | activation of isozyme BAA at 10 mM | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
x * 51000, isozyme BAA, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
isolate L1711, several isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular isozyme BAA from culture medium by ion exchange chromatographyand preparative PAGE | Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18.5 | - |
purified isozyme BAA | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | soluble starch | Bacillus sp. (in: Bacteria) | malto-oligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51000, isozyme BAA, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BAA | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
isozyme BAA | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
1 h, purified isozyme BAA, completely stable | Bacillus sp. (in: Bacteria) |
| 35 | - |
1 h, purified isozyme BAA, 84.3% remaining activity | Bacillus sp. (in: Bacteria) |
| 55 | - |
1 h, purified isozyme BAA, inactivation in presence of Ca2+, 1.6% residual activity in absence of Ca2+ | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | depends strongly on the temperature | Bacillus sp. (in: Bacteria) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | - |
Bacillus sp. (in: Bacteria) |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
