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Literature summary for 3.2.1.1 extracted from
- Temperature adaptation of proteins: engineering mesophilic-like activity and stability in a cold-adapted alpha-amylase (2003), J. Mol. Biol., 332, 981-988.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of AHA mutants in Escherichia coli | Pseudoalteromonas haloplanktis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K300R | 47% of wild-type kcat | Pseudoalteromonas haloplanktis |
| K300R/N150D/V196F/Q164I/T232V | 44% of wild-type kcat, increase in temperature stability | Pseudoalteromonas haloplanktis |
| N150D | 98% of wild-type kcat | Pseudoalteromonas haloplanktis |
| Q164I | 73% of wild-type kcat | Pseudoalteromonas haloplanktis |
| T232V | 105% of wild-type kcat | Pseudoalteromonas haloplanktis |
| V196F | 108% of wild-type kcat | Pseudoalteromonas haloplanktis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | 25°C, pH 7.2 | Sus scrofa |  |
| 0.23 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | 25°C, pH 7.2 | Pseudoalteromonas haloplanktis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Sus scrofa | - |
maltooligosaccharides | - |
? | |
| starch + H2O | Pseudoalteromonas haloplanktis | - |
maltooligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas haloplanktis | - |
- |
- |
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O | - |
Sus scrofa | 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose | - |
? | |
| 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O | - |
Pseudoalteromonas haloplanktis | 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose | - |
? | |
| starch + H2O | - |
Sus scrofa | maltooligosaccharides | - |
? | |
| starch + H2O | - |
Pseudoalteromonas haloplanktis | maltooligosaccharides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AHA | - |
Pseudoalteromonas haloplanktis |
| PPA | - |
Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
wild-type, approx. 70% loss of activity after 45 min in the absence of starch, approx. 95% loss of activity after 45 min in the presence of 2% starch, K300R/N150D/V196F/Q164I/T232V mutant, approx. 50% loss of activity in the absence of starch | Pseudoalteromonas haloplanktis |
| 60 | - |
approx. 50% loss of activity after 30 min in the presence of 2% starch, approx. 85% loss of activity after 30 min in the absence of starch | Sus scrofa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 141 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | 25°C, pH 7.2 | Sus scrofa | |
| 291 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | 25°C, pH 7.2 | Sus scrofa | |
| 392 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | 15°C, pH 7.2 | Pseudoalteromonas haloplanktis | |
| 697 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | 25°C, pH 7.2 | Pseudoalteromonas haloplanktis | |
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