Cloned (Comment) | Organism |
---|---|
expression in Bacillus subtilis | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
tiny needle crystals are formed in 35 mM Na-acetate, pH 4.6, 35 mM CaCl2, 6.25% 2-propanol in the presence of 1.23% of the pseudo-oligosaccharide inhibitor acarbose at a protein concentration of 10 mg/ml, crystals diffract to 2.0 A resolution | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-1,4-glucan + H2O | Geobacillus stearothermophilus | - |
fragments of alpha-1,4-glucan | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant alpha-amylase | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-1,4-glucan + H2O | - |
Geobacillus stearothermophilus | fragments of alpha-1,4-glucan | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BSTA | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
50% loss of activity after 50 min at pH 6.5 | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Geobacillus stearothermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
6.9 |