Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 4 mM, 30% inhibition | Saitozyma flava | |
Cu2+ | - |
Saitozyma flava | |
CuSO4 | 4 mM, complete inhibition | Saitozyma flava | |
Fe2+ | - |
Saitozyma flava | |
FeSO4 | 4 mM, 80% inhibition | Saitozyma flava | |
Hg2+ | - |
Saitozyma flava | |
HgCl2 | 4 mM, 71% inhibition | Saitozyma flava | |
additional information | treatment with glycosidases leads to loss of 50% activity | Saitozyma flava |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
starch | 0.056 mg/ml, 50°C, pH 5.5 | Saitozyma flava | |
0.057 | - |
soluble starch | pH 5.5, 50°C | Saitozyma flava |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Saitozyma flava | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
1 * 75000, SDS-PAGE | Saitozyma flava |
84500 | - |
gel filtration | Saitozyma flava |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Saitozyma flava | - |
maltose + maltotriose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saitozyma flava | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | highly glycosylated | Saitozyma flava |
glycoprotein | treatment with glycosidases leads to loss of 50% activity | Saitozyma flava |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme from culture medium 16.14fold in a single gel filtration step | Saitozyma flava |
Sephacryl S-100 | Saitozyma flava |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
842.9 units, one unit is defined as the amount of alpha-amylase necessary to hydrolyze 0.1 mg starch per min | Saitozyma flava |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Saitozyma flava | maltose + maltotriose | - |
? | |
amylopectin + H2O | - |
Saitozyma flava | maltose + maltotriose | main products | ? | |
amylose + H2O | - |
Saitozyma flava | maltose + maltotriose | - |
? | |
amylose + H2O | - |
Saitozyma flava | maltose + maltotriose | main products | ? | |
glycogen + H2O | - |
Saitozyma flava | maltose + maltotriose | main products | ? | |
glycogen starch + H2O | - |
Saitozyma flava | maltose + maltotriose | - |
? | |
pullulan + H2O | - |
Saitozyma flava | maltose + maltotriose | main products | ? | |
starch + H2O | - |
Saitozyma flava | maltose + maltotriose | - |
? | |
starch + H2O | soluble starch | Saitozyma flava | maltose + maltotriose | main products | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 75000, SDS-PAGE | Saitozyma flava |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Saitozyma flava |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10% loss of activity after 1 h | Saitozyma flava |
50 | - |
60 min, purified enzyme, 90% remaining activity | Saitozyma flava |
60 | - |
40% loss of activity after 1 h | Saitozyma flava |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Saitozyma flava |