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Literature summary for 3.2.1.1 extracted from
- Comparative studies on a mesophilic and a thermophilic alpha-amylase (2001), Appl. Biochem. Biotechnol., 90, 47-55.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dodecyltrimethylammonium bromide | 2.5 mM, approx. 40% inhibition at 60°C | Bacillus amyloliquefaciens |  |
| dodecyltrimethylammonium bromide | 2.5 mM, approx. 20% inhibition at 60°C | Bacillus licheniformis | |
| lauryl sulfobetaine | 2.5 mM, approx. 30% inhibition at 60°C | Bacillus amyloliquefaciens | |
| lauryl sulfobetaine | 2.5 mM, approx. 20% inhibition at 60°C | Bacillus licheniformis | |
| Sodium dodecyl sulfate | 2.5 mM, approx. 60% inhibition at 60°C | Bacillus amyloliquefaciens | |
| Sodium dodecyl sulfate | 2.5 mM, approx. 20% inhibition at 60°C | Bacillus licheniformis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Bacillus licheniformis | - |
fragments of starch | - |
? | |
| starch + H2O | Bacillus amyloliquefaciens | - |
fragments of starch | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| octane | strong protection against thermal inactivation | Bacillus licheniformis |
| octane | strong protection against thermal inactivation | Bacillus amyloliquefaciens |
| toluene | strong protection against thermal inactivation | Bacillus licheniformis |
| toluene | strong protection against thermal inactivation | Bacillus amyloliquefaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus amyloliquefaciens | - |
- |
- |
| Bacillus licheniformis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Bacillus licheniformis | fragments of starch | - |
? | |
| starch + H2O | - |
Bacillus amyloliquefaciens | fragments of starch | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 80 | - |
Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
30% loss of activity in the absence of additive, 21%, 5%, 7% and 10% loss of activity in the presence of 20% trehalose, sorbitol, mannitol and glycerol, respectively | Bacillus licheniformis |
| 80 | - |
96% loss of activity in the absence of additive, 93%, 80%, 86% and 91% loss of activity in the presence of 20% trehalose, sorbitol, mannitol and glycerol, respectively | Bacillus amyloliquefaciens |
| 90 | - |
no loss of activity in the presence of 50% toluene or n-octane | Bacillus licheniformis |
| 90 | - |
no loss of activity in the presence of 50% toluene or n-octane | Bacillus amyloliquefaciens |
| 95 | - |
approx. 50% loss of activity after 15 min, approx. 80% loss of activity after 15 min | Bacillus licheniformis |
| 95 | - |
approx. 50% loss of activity after 2 min, complete loss of activity after 20 min | Bacillus amyloliquefaciens |
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