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Literature summary for 3.2.1.1 extracted from
- Purification and properties of an alpha-amylase produced by a cassava-fermenting strain of Micrococcus luteus (1997), Folia Microbiol. (Praha), 42, 445-449.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Citric acid | 25 mM, 54% inhibition | Micrococcus luteus |  |
| EDTA | 25 mM, 92% inhibition | Micrococcus luteus | |
| KCN | 25 mM, 70% inhibition | Micrococcus luteus | |
| L-Cys | 25 mM, 26% inhibition | Micrococcus luteus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Micrococcus luteus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Micrococcus luteus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | optimal activation at 15 mM | Micrococcus luteus | |
| K+ | optimal activation at 10 mM | Micrococcus luteus | |
| Mg2+ | optimal activation at 10 mM | Micrococcus luteus | |
| Na+ | optimal activation at 5 mM | Micrococcus luteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
gel filtration | Micrococcus luteus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus luteus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Micrococcus luteus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Micrococcus luteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose + H2O | - |
Micrococcus luteus | additional information | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Micrococcus luteus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, 70% loss of activity, complete inactivation after 40 min | Micrococcus luteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Micrococcus luteus |
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Release 2023.1 (January 2023)
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