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Literature summary for 3.2.1.1 extracted from
- Physicochemical properties of a purified alpha-amylase from the thermophilic fungus Thermomyces lanuginosus (1992), Enzyme Microb. Technol., 14, 112-116.
No PubMed abstract available
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomyces lanuginosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermomyces lanuginosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Thermomyces lanuginosus | additional information | - |
? |  |
| glycogen + H2O | weak activity | Thermomyces lanuginosus | additional information | - |
? | |
| maltotriose + H2O | no activity | Thermomyces lanuginosus | maltose + D-glucose | - |
? | |
| starch + H2O | soluble starch | Thermomyces lanuginosus | additional information | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermomyces lanuginosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | about 60% of maximal activity at 40°C and at 70°C | Thermomyces lanuginosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Ca2+ protects against inactivation at 60°C | Thermomyces lanuginosus |
| 50 | - |
at pH 6.0-7.5 stable for at least 20 h without substrate and Ca2+, incubation at pH 8.0 under the same conditions results in 40% decrease in activity | Thermomyces lanuginosus |
| 60 | - |
half-life: 140 min | Thermomyces lanuginosus |
| 70 | - |
half-life: 10 min | Thermomyces lanuginosus |
| 80 | - |
5 min, complete inactivation | Thermomyces lanuginosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | 5.2 | - |
Thermomyces lanuginosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | about 35% of maximal activity at pH 4.0 and at pH 7.0 | Thermomyces lanuginosus |
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Release 2023.1 (January 2023)
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