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Literature summary for 3.2.1.1 extracted from

  • Terashima, M.; Kawai, M.; Kumagai, M.H.; Rodriguez, R.L.; Katoh, S.
    Characteristics of a chimeric enzyme engineered from two rice alpha-amylase isozymes (1996), Appl. Microbiol. Biotechnol., 45, 607-611.
No PubMed abstract available
Search for more literature for 3.2.1.1

Protein Variants

Protein Variants Comment Organism
additional information chimeric enzyme engineered from two rice alpha-amylases isoenzymes, Amy1A and Amy3D. Amy1 shows high activity in soluble-starch hydrolysis and low activity in oligosaccharide degradation, while Amy3D shows low activity in soluble-starch hydrolysis and high activity in oligosaccharide degradation. The chimeric enzyme shows high activities in both soluble-starch hydrolysis and oligosaccharide degradation Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose + H2O
-
 Oryza sativa additional information
-
 ?
additional information chimeric enzyme engineered from two rice alpha-amylases isoenzymes, Amy1A and Amy3D. Amy1 shows high activity in soluble-starch hydrolysis and low activity in oligosaccharide degradation, while Amy3D shows low activity in soluble-starch hydrolysis and high activity in oligosaccharide degradation. The chimeric enzyme shows high activities in both soluble-starch hydrolysis and oligosaccharide degradation Oryza sativa ?
-
 ?
starch + H2O soluble starch Oryza sativa additional information
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 15 min, chimeric enzyme, 45% loss of activity Oryza sativa
78
-
 5 min, chimeric enzyme, about 90% loss of activity Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5 isoenzyme Amy1A Oryza sativa
5
-
 isoenzyme Amy3D Oryza sativa