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Literature summary for 3.2.1.1 extracted from

  • Igarashi, K.; Hatada, Y.; Hagihara, H.; Saeki, K.; Takaiwa, M.; Uemura, T.; Ara, K.; Ozaki, K.; Kawai, S.; Kobayashi, T.; Ito, S.
    Enzymatic properties of a novel liquefying alpha-amylase from an alkaliphilic Bacillus isolate and entire nucleotide and amino acid sequences (1998), Appl. Environ. Microbiol., 64, 3282-3289.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM, 91% inhibition Bacillus sp. (in: Bacteria)
EDTA 10 mM, 90% inhibition Bacillus sp. (in: Bacteria)
EGTA 10 mM, 91% inhibition Bacillus sp. (in: Bacteria)
Hg2+ 1 mM, complete inhibition Bacillus sp. (in: Bacteria)
iodoacetate 0.5 mM, 98% inhibition Bacillus sp. (in: Bacteria)
N-bromosuccinimide 0.1 mM, complete inhibition Bacillus sp. (in: Bacteria)
Ni2+ 1 mM, 82% inhibition Bacillus sp. (in: Bacteria)
PCMB 0.5 mM, 20% inhibition Bacillus sp. (in: Bacteria)
Zn2+ 1 mM, complete inhibition Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.89
-
maltooctaose
-
Bacillus sp. (in: Bacteria)
2.18
-
maltoheptaose
-
Bacillus sp. (in: Bacteria)
6.37
-
maltohexaose
-
Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
x * 53000, extracellular enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)
55391
-
x * 55391, extracellular enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
KSM-1378
-
Bacillus sp. (in: Bacteria) KSM-1378
-
KSM-1378
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5009
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Bacillus sp. (in: Bacteria) additional information
-
?
amylopectin + H2O
-
Bacillus sp. (in: Bacteria) KSM-1378 additional information
-
?
amylose + H2O
-
Bacillus sp. (in: Bacteria) additional information
-
?
amylose + H2O
-
Bacillus sp. (in: Bacteria) KSM-1378 additional information
-
?
glycogen + H2O
-
Bacillus sp. (in: Bacteria) additional information
-
?
maltodextran + H2O no activity Bacillus sp. (in: Bacteria) ?
-
?
maltodextrin + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
maltoheptaose + H2O
-
Bacillus sp. (in: Bacteria) additional information
-
?
maltohexaose + H2O
-
Bacillus sp. (in: Bacteria) additional information glucose + maltopentaose ?
maltooctaose + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
maltopentaose + H2O no activity Bacillus sp. (in: Bacteria) additional information
-
?
maltotetraose + H2O no activity Bacillus sp. (in: Bacteria) additional information
-
?
maltotriose + H2O no activity Bacillus sp. (in: Bacteria) maltose + D-glucose
-
?
maltotriose + H2O no activity Bacillus sp. (in: Bacteria) KSM-1378 maltose + D-glucose
-
?
pullulan + H2O no activity Bacillus sp. (in: Bacteria) panose + ?
-
?
starch + H2O soluble starch Bacillus sp. (in: Bacteria) additional information main products in the early stage of hydrolysis are maltopentaose, maltohexaose and maltoheptaose, on further incubation maltotriose and maltopentaose increase while maltohexaose, maltoheptaose and maltooctaose decrease ?
starch + H2O soluble starch Bacillus sp. (in: Bacteria) additional information major products: maltotriose + maltopentaose + maltohexaose + maltose ?
starch + H2O soluble starch Bacillus sp. (in: Bacteria) KSM-1378 additional information main products in the early stage of hydrolysis are maltopentaose, maltohexaose and maltoheptaose, on further incubation maltotriose and maltopentaose increase while maltohexaose, maltoheptaose and maltooctaose decrease ?
starch + H2O soluble starch Bacillus sp. (in: Bacteria) KSM-1378 additional information major products: maltotriose + maltopentaose + maltohexaose + maltose ?

Subunits

Subunits Comment Organism
? x * 53000, extracellular enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)
? x * 55391, extracellular enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
LAMY
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30°C: about 40% of maximal activity, 80°C: about 30% of maximal activity Bacillus sp. (in: Bacteria)
40 70 40°C: about 70% of maximal activity, 70°C: about 75% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
pH 8.5, 20 mM Tris-HCl buffer, in absence of CaCl2, stable for 60 min Bacillus sp. (in: Bacteria)
50
-
pH 8.5, 20 mM Tris-HCl buffer, in absence of CaCl2, 68% loss of activity, stable in presence of 0.2 mM CaCl2 Bacillus sp. (in: Bacteria)
60
-
pH 8.5, 20 mM Tris-HCl buffer, in absence of CaCl2, 97% loss of activity, 35% loss of activity in presence of 0.1 mM CaCl2 Bacillus sp. (in: Bacteria)
80
-
complete inactivation after 60 min in presence of 0.1 mM Ca2+ Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.8 pH 4.5: about 30% of maximal activity, pH 9.8: about 40% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 40°C, 30 min, 50 mM Tris-HCl buffer, stable Bacillus sp. (in: Bacteria)