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Literature summary for 3.2.1.1 extracted from
- Purification and some properties of an extracellular alpha-amylase from Bacteroides amylophilus (1977), J. Bacteriol., 129, 1537-1544.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-phenylglucoside | - |
Ruminobacter amylophilus |  |
| cyclomaltoheptaose | - |
Ruminobacter amylophilus | |
| cyclomaltohexaose | - |
Ruminobacter amylophilus | |
| EDTA | 0.01 M, 58% loss of activity | Ruminobacter amylophilus | |
| Hg2+ | 5 mM, 85% inhibition | Ruminobacter amylophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Ruminobacter amylophilus | |
| 16.7 | - |
Dextrin | micromol of D-glucosidic bond per liter | Ruminobacter amylophilus | |
| 18.2 | - |
amylose | micromol of D-glucosidic bond per liter | Ruminobacter amylophilus | |
| 18.2 | - |
amylopectin | micromol of D-glucosidic bond per liter | Ruminobacter amylophilus | |
| 18.7 | - |
starch | micromol of D-glucosidic bond per L | Ruminobacter amylophilus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Ruminobacter amylophilus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| Ca2+ | 5 mM, about 70% activation | Ruminobacter amylophilus | |
| Co2+ | 5 mM, about 70% activation | Ruminobacter amylophilus | |
| Cu2+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| Fe2+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| K+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| Li+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| Mg2+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| Na+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| Pb2+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| Zn2+ | 5 mM, slight activation | Ruminobacter amylophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
x * 92000, SDS-PAGE | Ruminobacter amylophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruminobacter amylophilus | - |
- |
- |
| Ruminobacter amylophilus 70 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ruminobacter amylophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 122 | - |
- |
Ruminobacter amylophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | 92% of the activity with amylose | Ruminobacter amylophilus | additional information | - |
? | |
| amylopectin + H2O | 92% of the activity with amylose | Ruminobacter amylophilus 70 | additional information | - |
? | |
| amylose + H2O | - |
Ruminobacter amylophilus | additional information | - |
? | |
| amylose + H2O | - |
Ruminobacter amylophilus 70 | additional information | - |
? | |
| dextrin + H2O | - |
Ruminobacter amylophilus | ? | - |
? | |
| dextrin + H2O | - |
Ruminobacter amylophilus 70 | ? | - |
? | |
| maltodextrin + H2O | 60% of the activity with amylose | Ruminobacter amylophilus | ? | - |
? | |
| maltotetraose + H2O | - |
Ruminobacter amylophilus | additional information | maltose + maltose | ? | |
| maltotetraose + H2O | - |
Ruminobacter amylophilus 70 | additional information | maltose + maltose | ? | |
| starch + H2O | soluble starch | Ruminobacter amylophilus | additional information | products in the late stage of reaction: maltose + maltotriose with less amounts of maltotetraose and maltopentaose and traces of glucose | ? | |
| starch + H2O | soluble starch | Ruminobacter amylophilus 70 | additional information | products in the late stage of reaction: maltose + maltotriose with less amounts of maltotetraose and maltopentaose and traces of glucose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 92000, SDS-PAGE | Ruminobacter amylophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 43 | - |
- |
Ruminobacter amylophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | 25°C, about 50% of maximal activity, 50°C: about 85% of maximal activity | Ruminobacter amylophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Ruminobacter amylophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | about 45% of maximal activity at pH 5.0 and at pH 8.0 | Ruminobacter amylophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 7.5 | 25°C, 0.067 M phosphate buffer, enzyme retains over 93% of its activity | Ruminobacter amylophilus |
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