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Literature summary for 3.2.1.1 extracted from
- Purification and characterization of alpha-amylase from Bacillus licheniformis CUMC305 (1983), Appl. Environ. Microbiol., 46, 430-437.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Bacillus licheniformis |  |
| Al3+ | - |
Bacillus licheniformis | |
| Cd2+ | - |
Bacillus licheniformis | |
| Co2+ | - |
Bacillus licheniformis | |
| Cu2+ | - |
Bacillus licheniformis | |
| EDTA | resistant at 30°C, inhibition at 90°C, inhibition is partially recovered by Cu2+ or Fe2+ | Bacillus licheniformis | |
| Fe2+ | - |
Bacillus licheniformis | |
| Hg2+ | partially reversed by Cys | Bacillus licheniformis | |
| iodoacetate | around 100 mM | Bacillus licheniformis | |
| Mn2+ | - |
Bacillus licheniformis | |
| Ni2+ | - |
Bacillus licheniformis | |
| PCMB | - |
Bacillus licheniformis | |
| Zn2+ | - |
Bacillus licheniformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Bacillus licheniformis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates | Bacillus licheniformis | |
| F- | stimulates | Bacillus licheniformis | |
| Mg2+ | stimulates | Bacillus licheniformis | |
| MoO42- | 10-100 mM, stimulates | Bacillus licheniformis | |
| Na+ | stimulates | Bacillus licheniformis | |
| PO43- | 100 mM, stimulates | Bacillus licheniformis | |
| S2O32- | stimulates | Bacillus licheniformis | |
| SO32- | stimulates | Bacillus licheniformis | |
| SO42- | stimulates | Bacillus licheniformis | |
| WO42- | stimulates | Bacillus licheniformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, SDS-PAGE | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
| Bacillus licheniformis CUMC305 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus licheniformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Bacillus licheniformis | additional information | - |
? | |
| amylopectin + H2O | - |
Bacillus licheniformis CUMC305 | additional information | - |
? | |
| amylose + H2O | - |
Bacillus licheniformis | additional information | - |
? | |
| amylose + H2O | - |
Bacillus licheniformis CUMC305 | additional information | - |
? | |
| glycogen + H2O | - |
Bacillus licheniformis | additional information | - |
? | |
| glycogen + H2O | - |
Bacillus licheniformis CUMC305 | additional information | - |
? | |
| starch + H2O | soluble starch | Bacillus licheniformis | additional information | - |
? | |
| starch + H2O | soluble starch | Bacillus licheniformis CUMC305 | additional information | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
3 h, in absence of substrate, 9% loss of activity | Bacillus licheniformis |
| 70 | - |
3 h, in absence of substrate, 21% loss of activity | Bacillus licheniformis |
| 80 | - |
90 min, in absence of substrate, 29% loss of activity | Bacillus licheniformis |
| 100 | - |
4 h, in presence of starch, completely stable | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
30°C, 18 h, 50% loss of activity | Bacillus licheniformis |
| 4 | - |
30°C, 18 h, 34% loss of activity | Bacillus licheniformis |
| 5 | - |
30°C, 18 h, 25% loss of activity | Bacillus licheniformis |
| 6 | - |
30°C, 18 h, 15% loss of activity | Bacillus licheniformis |
| 7 | 9 | 30°C, 18 h, stable | Bacillus licheniformis |
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