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Literature summary for 3.13.1.4 extracted from
- A novel 3-sulfinopropionyl coenzyme A (3SP-CoA) desulfinase from Advenella mimigardefordensis strain DPN7T acting as a key enzyme during catabolism of 3,3'-dithiodipropionic acid is a member of the acyl-CoA dehydrogenase superfamily (2013), J. Bacteriol., 195, 1538-1551.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | the enzyme is part of the 3,3'-dithiopropionate degradation pathway. The elucidation of this pathway and the identification of the genes could provide an strategy to engineer strains suitable for biotechnological production of polythioesters | Advenella mimigardefordensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologously expressed in Escherichia coli BL21(DE3)/pLysS by using apET23a::acdDPN7 vector | Advenella mimigardefordensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTAacd | the mutant is defective in growth on 3,3'-dithiopropionate and 3-sulfinopropionate | Advenella mimigardefordensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C, with a Vmax of 4.19 micromol/min*mg | Advenella mimigardefordensis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43531 | - |
4 * 43531, calculated, 4 * 44809, His6-tag, calculated | Advenella mimigardefordensis |
| 44809 | - |
4 * 43531, calculated, 4 * 44809, His6-tag, calculated | Advenella mimigardefordensis |
| 173000 | - |
gel filtration of the native protein, indicating a homotetrameric structure, in accordance with other members of the acyl-CoA dehydrogenase superfamily, in accordance to the theoretical molecular mass of 179200 Da | Advenella mimigardefordensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-sulfinopropionyl-CoA + H2O | Advenella mimigardefordensis | - |
propionyl-CoA + sulfite | analyzed by HPLC-ESI-MS | ? | |
| 3-sulfinopropionyl-CoA + H2O | Advenella mimigardefordensis DPN7T | - |
propionyl-CoA + sulfite | analyzed by HPLC-ESI-MS | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Advenella mimigardefordensis | - |
betaproteobacterium | - |
| Advenella mimigardefordensis DPN7T | - |
betaproteobacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His6-tagged recombinant protein | Advenella mimigardefordensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-sulfinopropionyl-CoA + H2O | - |
Advenella mimigardefordensis | propionyl-CoA + sulfite | analyzed by HPLC-ESI-MS | ? | |
| 3-sulfinopropionyl-CoA + H2O | - |
Advenella mimigardefordensis DPN7T | propionyl-CoA + sulfite | analyzed by HPLC-ESI-MS | ? | |
| additional information | the enzyme is unable to perform a dehydrogenation, which is the usual reaction catalyzed by members of the acyl-CoA dehydrogenase superfamily | Advenella mimigardefordensis | ? | - |
? | |
| additional information | the enzyme is unable to perform a dehydrogenation, which is the usual reaction catalyzed by members of the acyl-CoA dehydrogenase superfamily | Advenella mimigardefordensis DPN7T | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 43531, calculated, 4 * 44809, His6-tag, calculated | Advenella mimigardefordensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3SP-CoA desulfinase | - |
Advenella mimigardefordensis |
| AcdDPN7 | - |
Advenella mimigardefordensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Advenella mimigardefordensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.13 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C | Advenella mimigardefordensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Advenella mimigardefordensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | the purified protein is yellow and contains a non-covalently bound FAD, verified by HPLC-ESI-MS analysis | Advenella mimigardefordensis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Advenella mimigardefordensis | including His6-tag, calculated | - |
6.71 |
| Advenella mimigardefordensis | calculated | - |
6.73 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is responsilbe for the final defulfination step during catabolism of 3,3'-dithiopropionate, a sulfur-containing substrate for biosynthensis of polythioesters, the final reaction before propionyl-CoA enters the central metabolism on the level of the methylcitric acid cycle | Advenella mimigardefordensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 240.8 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C | Advenella mimigardefordensis | |
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Release 2023.1 (January 2023)
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