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Literature summary for 3.13.1.3 extracted from

  • Ohshiro, T.; Ohkita, R.; Takikawa, T.; Manabe, M.; Lee, W.C.; Tanokura, M.; Izumi, Y.
    Improvement of 2-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis (2007), Biosci. Biotechnol. Biochem., 71, 2815-2821.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodococcus erythropolis

Protein Variants

Protein Variants Comment Organism
Q65H increase in thermostability, decrease in catalytic activity Rhodococcus erythropolis
Y63A increase in catalytic activity Rhodococcus erythropolis
Y63F increase in catalytic activity, similar thermostability as wild-type Rhodococcus erythropolis
Y63F/Q65H increase in thermostability without loss of catalytic activity or affinity for substrate Rhodococcus erythropolis
Y63K decrease in activity Rhodococcus erythropolis
Y63S affinity for the substrate is about 7-fold lower than that of the wild-type enzyme Rhodococcus erythropolis
Y63W activity similar to wild-type Rhodococcus erythropolis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0056
-
 2'-hydroxybiphenyl-2-sulfinate mutant Y63F, pH 7.0, 28°C Rhodococcus erythropolis
0.0082
-
 2'-hydroxybiphenyl-2-sulfinate wild-type, pH 7.0, 28°C Rhodococcus erythropolis
0.0098
-
 2'-hydroxybiphenyl-2-sulfinate mutant Y63F/Q65H, pH 7.0, 28°C Rhodococcus erythropolis
0.0556
-
 2'-hydroxybiphenyl-2-sulfinate mutant Y63S, pH 7.0, 28°C Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis P54997 strain KA2-5-1
-
Rhodococcus erythropolis IGTS8 / ATCC 53968 P54997 strain KA2-5-1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.3
-
 mutant Y63K, pH 7.0, 28°C Rhodococcus erythropolis
21
-
 mutant Q65H, pH 7.0, 28°C Rhodococcus erythropolis
26.8
-
 wild-type, pH 7.0, 28°C Rhodococcus erythropolis
29.2
-
 mutant Y63W, pH 7.0, 28°C Rhodococcus erythropolis
34.1
-
 mutant Y63F/Q65H, pH 7.0, 28°C Rhodococcus erythropolis
43
-
 mutant Y63F, pH 7.0, 28°C Rhodococcus erythropolis
49.1
-
 mutant Y63A, pH 7.0, 28°C Rhodococcus erythropolis
61.1
-
 mutant Y63S, pH 7.0, 28°C Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-hydroxybiphenyl-2-sulfinate + H2O
-
 Rhodococcus erythropolis 2-hydroxybiphenyl + sulfite
-
 ?
2'-hydroxybiphenyl-2-sulfinate + H2O
-
 Rhodococcus erythropolis IGTS8 / ATCC 53968 2-hydroxybiphenyl + sulfite
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 30 min, wild-type, almost complete loss of activity, mutant Y63F/Q65H, 90% residual activity Rhodococcus erythropolis
40
-
 30 min,mutant Y63F/Q65H, 90% residual activity Rhodococcus erythropolis