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Literature summary for 3.13.1.3 extracted from

  • Konishi, J.; Maruhashi, K.
    2-(2'-Hydroxyphenyl)benzene sulfinate desulfinase from the thermophilic desulfurizing bacterium Paenibacillus sp. strain A11-2: purification and characterization (2003), Appl. Microbiol. Biotechnol., 62, 356-361.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Mg2+ 1.23fold activation at 1 mM Paenibacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene tdsB, DNA sequence determination, overexpression in Escherichia coli BL21 (DE3) Paenibacillus sp.

Protein Variants

Protein Variants Comment Organism
C17S site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant Paenibacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxybiphenyl 30% inhibition at 5 mM, the OH-group is important for the inhibitory effect Paenibacillus sp.
Cu2+ 67% inhibition at 1 mM Paenibacillus sp.
DTNB 86% inhibition at 0.1 mM Paenibacillus sp.
Fe2+ 44% inhibition at 1 mM Paenibacillus sp.
iodoacetic acid 95% inhibition at 1 mM Paenibacillus sp.
Mn2+ 71% inhibition at 1 mM Paenibacillus sp.
additional information no inhibition by 1,10-phenanthroline and EDTA, no inhibition by Co2+, Ca2+, and Mg2+, no inhibition by biphenyl, 2-methylbiphenyl, and 2-methoxybiphenyl Paenibacillus sp.
N-ethylmaleimide complete inhibition at 1 mM Paenibacillus sp.
Ni2+ 24% inhibition at 1 mM Paenibacillus sp.
o-hydroxystyrene product inhibition, 80% inhibition at 5 mM, the OH-group is important for the inhibitory effect Paenibacillus sp.
p-chloromercuribenzoate 92% inhibition at 0.1 mM Paenibacillus sp.
Zn2+ 98% inhibition at 1 mM Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
2-(2-hydroxyphenyl)benzenesulfinate pH 7.0, 50°C Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
gel filtration Paenibacillus sp.
39000
-
1 * 39000, SDS-PAGE Paenibacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-(2'-hydroxyphenyl)benzenesulfinate + H2O Paenibacillus sp. final step of the desulfurozation of dibenzothiophene 2-hydroxybiphenyl + sulfite
-
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O Paenibacillus sp. A11-2 final step of the desulfurozation of dibenzothiophene 2-hydroxybiphenyl + sulfite
-
ir

Organism

Organism UniProt Comment Textmining
Paenibacillus sp.
-
-
-
Paenibacillus sp. A11-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain A11-2 63fold Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.139
-
purified enzyme Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
-
Paenibacillus sp. 2-hydroxybiphenyl + sulfite
-
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O final step of the desulfurozation of dibenzothiophene Paenibacillus sp. 2-hydroxybiphenyl + sulfite
-
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
-
Paenibacillus sp. A11-2 2-hydroxybiphenyl + sulfite
-
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O final step of the desulfurozation of dibenzothiophene Paenibacillus sp. A11-2 2-hydroxybiphenyl + sulfite
-
ir
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O
-
Paenibacillus sp. o-hydroxystyrene + sulfite
-
?
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O
-
Paenibacillus sp. A11-2 o-hydroxystyrene + sulfite
-
?

Subunits

Subunits Comment Organism
monomer 1 * 39000, SDS-PAGE Paenibacillus sp.

Synonyms

Synonyms Comment Organism
2-(2'-hydroxyphenyl)benzene sulfinate desulfinase
-
Paenibacillus sp.
TdsB
-
Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
30 min, stable up to Paenibacillus sp.
60
-
rapid loss of activity Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Paenibacillus sp.

Cofactor

Cofactor Comment Organism Structure
additional information no cofactors required Paenibacillus sp.