Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion results in bipyramidal crystals, C-2-symmetric homodimer, the protein has a bidomain structure, catalytic site is located in the cleft formed between the two lobes | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the function of different amino acids in reaction/binding process is analysed, Thr-145, Tyr-182 and Lys-186 are found to fulfill analogous mechanistic roles | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | thylakoid | Arabidopsis thaliana | 9507 | - |
membrane | photosynthetic | Arabidopsis thaliana | 16020 | - |
thylakoid | - |
Arabidopsis thaliana | 9579 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + sulfite | Arabidopsis thaliana | sulfolipid biosynthesis | UDP-sulfoquinovose + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O48917 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-nitrilotriacetic acid column | Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite | contains characteristic conserved Y-XXX-K and glycine-rich sequence patterns | Arabidopsis thaliana | |
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite | short-chain dehydrogenase/reductase family | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + sulfite | - |
Arabidopsis thaliana | UDP-sulfoquinovose + H2O | - |
? | |
UDP-glucose + sulfite | sulfolipid biosynthesis | Arabidopsis thaliana | UDP-sulfoquinovose + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana |