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Literature summary for 3.11.1.3 extracted from

  • Ternan, N.G.; Hamilton, J.T.; Quinn, J.P.
    Initial in vitro characterisation of phosphonopyruvate hydrolase, a novel phosphate starvation-independent, carbon-phosphorus bond cleavage enzyme in Burkholderia cepacia Pal6 (2000), Arch. Microbiol., 173, 35-41.
    View publication on PubMed

General Stability

General Stability Organism
dialysis of cell-free extract for 24 h at 4°C results in a 72% loss of activity Burkholderia cepacia

Inhibitors

Inhibitors Comment Organism Structure
3-phosphonopropionic acid 10 mM, 21% inhibition Burkholderia cepacia
Aminomethylphosphonic acid 10 mM, 19% inhibition Burkholderia cepacia
EDTA Co2+, Ni2+, Mg2+, Zn2+, Fe3+ and Ca2+ recover activity. Cu2+, Mn2+ and Cs+ do not recover activity Burkholderia cepacia
Fe2+ 5 mM, 94% inhibition Burkholderia cepacia
glyphosate 10 mM, 23% inhibition Burkholderia cepacia
Mn2+ 5 mM, 12% inhibition Burkholderia cepacia
Phosphonoacetate 10 mM, 30% inhibition Burkholderia cepacia
phosphonobutyric acid 10 mM, 14% inhibition Burkholderia cepacia
Phosphonoformate 10 mM, 37% inhibition Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.72
-
3-phosphonopyruvate
-
Burkholderia cepacia

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 1.5fold activation Burkholderia cepacia
Co2+ 5 mM, 4.8fold activation Burkholderia cepacia
Cu2+ 5 mM, 1.2fold activation Burkholderia cepacia
Mg2+ 5 mM, 3.4fold activation Burkholderia cepacia
Ni2+ 5 mM, 4.3fold activation Burkholderia cepacia
Zn2+ 5 mM, 1.8fold activation Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
232000
-
non-denaturing PAGE Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-phosphonopyruvate + H2O Burkholderia cepacia the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction pyruvate + phosphate
-
?
3-phosphonopyruvate + H2O Burkholderia cepacia Pal6 the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction pyruvate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-
Burkholderia cepacia Pal6
-
-
-

Storage Stability

Storage Stability Organism
-20°C, 10 weeks, 54% loss of activity Burkholderia cepacia
20, 50% loss of activity after 3 days, complete loss of activity after 6 days Burkholderia cepacia
4°C, 30 days, 38% loss of activity Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phosphonopyruvate + H2O
-
Burkholderia cepacia pyruvate + phosphate
-
?
3-phosphonopyruvate + H2O the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction Burkholderia cepacia pyruvate + phosphate
-
?
3-phosphonopyruvate + H2O
-
Burkholderia cepacia Pal6 pyruvate + phosphate
-
?
3-phosphonopyruvate + H2O the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction Burkholderia cepacia Pal6 pyruvate + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Burkholderia cepacia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Burkholderia cepacia