General Stability | Organism |
---|---|
dialysis of cell-free extract for 24 h at 4°C results in a 72% loss of activity | Burkholderia cepacia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-phosphonopropionic acid | 10 mM, 21% inhibition | Burkholderia cepacia | |
Aminomethylphosphonic acid | 10 mM, 19% inhibition | Burkholderia cepacia | |
EDTA | Co2+, Ni2+, Mg2+, Zn2+, Fe3+ and Ca2+ recover activity. Cu2+, Mn2+ and Cs+ do not recover activity | Burkholderia cepacia | |
Fe2+ | 5 mM, 94% inhibition | Burkholderia cepacia | |
glyphosate | 10 mM, 23% inhibition | Burkholderia cepacia | |
Mn2+ | 5 mM, 12% inhibition | Burkholderia cepacia | |
Phosphonoacetate | 10 mM, 30% inhibition | Burkholderia cepacia | |
phosphonobutyric acid | 10 mM, 14% inhibition | Burkholderia cepacia | |
Phosphonoformate | 10 mM, 37% inhibition | Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.72 | - |
3-phosphonopyruvate | - |
Burkholderia cepacia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 1.5fold activation | Burkholderia cepacia | |
Co2+ | 5 mM, 4.8fold activation | Burkholderia cepacia | |
Cu2+ | 5 mM, 1.2fold activation | Burkholderia cepacia | |
Mg2+ | 5 mM, 3.4fold activation | Burkholderia cepacia | |
Ni2+ | 5 mM, 4.3fold activation | Burkholderia cepacia | |
Zn2+ | 5 mM, 1.8fold activation | Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
232000 | - |
non-denaturing PAGE | Burkholderia cepacia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phosphonopyruvate + H2O | Burkholderia cepacia | the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction | pyruvate + phosphate | - |
? | |
3-phosphonopyruvate + H2O | Burkholderia cepacia Pal6 | the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction | pyruvate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
- |
- |
Burkholderia cepacia Pal6 | - |
- |
- |
Storage Stability | Organism |
---|---|
-20°C, 10 weeks, 54% loss of activity | Burkholderia cepacia |
20, 50% loss of activity after 3 days, complete loss of activity after 6 days | Burkholderia cepacia |
4°C, 30 days, 38% loss of activity | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phosphonopyruvate + H2O | - |
Burkholderia cepacia | pyruvate + phosphate | - |
? | |
3-phosphonopyruvate + H2O | the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction | Burkholderia cepacia | pyruvate + phosphate | - |
? | |
3-phosphonopyruvate + H2O | - |
Burkholderia cepacia Pal6 | pyruvate + phosphate | - |
? | |
3-phosphonopyruvate + H2O | the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction | Burkholderia cepacia Pal6 | pyruvate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Burkholderia cepacia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Burkholderia cepacia |