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Literature summary for 3.1.8.2 extracted from
- Catalytic efficiencies of directly evolved phosphotriesterase variants with structurally different organophosphorus compounds in vitro (2016), Arch. Toxicol., 90, 2711-2724 .
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | the organophosphorus-hydrolyzing enzymes, such as Brevundimonas diminuta phosphotriesterase (PTE), are used for development of alternative catalytic bioscavengers to treat organophosphorus agents, e.g. pesticides and warfare agents, intoxications of humans | Brevundimonas diminuta |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene opd, recombinant expression of wild-type and mutant MBP-tagged enzymes in Escherichia coli strain BL21(DE3) | Brevundimonas diminuta |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | a PTE mutant dubbed C23 is engineered, exhibiting reversed stereoselectivity and high catalytic efficiency (kcat/KM) for the hydrolysis of the toxic enantiomers of nerve agents VX, CVX, and VR. The other mutants A53, IV-A1, IV-H3, B141, and RD1-G83 are less effective, activities and mutant-substrate docking, overview. The only exception is IV-A1 with amiton. Most variants are highly efficient when hydrolyzing N,N-diisopropyl substrates and are 2fold less efficient with N,N-diethyl substrates and 5fold less efficient with N,N-dimethyl substrates | Brevundimonas diminuta |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | reaction kinetics of wild-type and mutant enzymes, overview | Brevundimonas diminuta |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required | Brevundimonas diminuta |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevundimonas diminuta | P0A434 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (RS)-propan-2-yl methylphosphonofluoridate + H2O | i.e. sarin | Brevundimonas diminuta | propan-2-yl methylphosphonate + fluoride | - |
? | |
| 3-[fluoro(methyl)phosphoryl]oxy-2,2-dimethylbutane + H2O | i.e. soman | Brevundimonas diminuta | 3,3-dimethylbutan-2-yl methylphosphonate + fluoride | - |
? | |
| ethyl dimethylphosphoramidocyanidate + H2O | i.e. tabun | Brevundimonas diminuta | ? | - |
? | |
| ethyl dimethylphosphoramidocyanidate + H2O | i.e. tabun | Brevundimonas diminuta | ethyl hydrogen dimethylphosphoramidate + HCN | - |
? | |
| additional information | the enzyme also exhibits activity of EC 3.1.8.1, aryldialkylphosphatase, as well as of EC 3.1.8.2, diisopropyl-fluorophosphatase. Enzyme-substrate docking study, overview | Brevundimonas diminuta | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | cf. EC 3.1.8.1 | Brevundimonas diminuta |
| OP-hydrolyzing enzyme | - |
Brevundimonas diminuta |
| opd | - |
Brevundimonas diminuta |
| organophosphorus-hydrolyzing enzyme | - |
Brevundimonas diminuta |
| PTES5 | - |
Brevundimonas diminuta |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Brevundimonas diminuta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Brevundimonas diminuta |
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Release 2023.1 (January 2023)
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