Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.8.2 extracted from

  • Melzer, M.; Chen, J.C.; Heidenreich, A.; Gaeb, J.; Koller, M.; Kehe, K.; Blum, M.M.
    Reversed enantioselectivity of diisopropyl fluorophosphatase against organophosphorus nerve agents by rational design (2009), J. Am. Chem. Soc., 131, 17226-17232.
    View publication on PubMed
Search for more literature for 3.1.8.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Loligo vulgaris

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzymes are crystallized at room temperature by hanging drop vapor diffusion method, using 0.1 M Tris buffer pH 8.5, 2% tacsimate, 16% (w/v) PEG 3350 for mutant E37A/Y144A/R146A/T195M or 0.2 M KCl, 0.05 M HEPES buffer pH 7.5, 35% (w/v) pentaerythriol propoxylate for mutant E37D/Y144A/R16A/T195M Loligo vulgaris

Protein Variants

Protein Variants Comment Organism
E37A/Y144A/R146A/T195M the mutant shows increased turnover number and kcat/Km for diisopropyl fluorophosphate compared to the wild type enzyme Loligo vulgaris
E37D/Y144A/R16A/T195M the mutant shows increased turnover number and kcat/Km for diisopropyl fluorophosphate compared to the wild type enzyme Loligo vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.12
-
 diisopropyl fluorophosphate mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
3.76
-
 diisopropyl fluorophosphate wild type enzyme Loligo vulgaris
3.93
-
 diisopropyl fluorophosphate mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris

Organism

Organism UniProt Comment Textmining
Loligo vulgaris Q7SIG4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Loligo vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-cyclosarin + H2O
-
 Loligo vulgaris ?
-
 ?
(R)-sarin + H2O
-
 Loligo vulgaris ?
-
 ?
(S)-cyclosarin + H2O
-
 Loligo vulgaris ?
-
 ?
(S)-sarin + H2O
-
 Loligo vulgaris ?
-
 ?
diisopropyl fluorophosphate + H2O
-
 Loligo vulgaris diisopropyl phosphate + fluoride
-
 ?
soman + H2O
-
 Loligo vulgaris ?
-
 ?

Synonyms

Synonyms Comment Organism
DFPase
-
 Loligo vulgaris
diisopropyl fluorophosphatase
-
 Loligo vulgaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
211
-
 diisopropyl fluorophosphate wild type enzyme Loligo vulgaris
291
-
 diisopropyl fluorophosphate mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
376
-
 diisopropyl fluorophosphate mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17
-
 (S)-cyclosarin wild type enzyme Loligo vulgaris
24
-
 (R)-sarin mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris
24
-
 (S)-cyclosarin mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris
27
-
 (R)-cyclosarin mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris
42
-
 (S)-sarin wild type enzyme Loligo vulgaris
45
-
 (R)-sarin mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
47
-
 (R)-sarin wild type enzyme Loligo vulgaris
56
-
 diisopropyl fluorophosphate wild type enzyme Loligo vulgaris
71
-
 (S)-sarin mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris
96
-
 diisopropyl fluorophosphate mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris
130
-
 (R)-cyclosarin mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
140
-
 diisopropyl fluorophosphate mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
230
-
 (S)-sarin mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
490
-
 (S)-cyclosarin mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
720
-
 (R)-cyclosarin wild type enzyme Loligo vulgaris