Crystallization (Comment) | Organism |
---|---|
in complex with inhibitor O,O-dicyclopentylphosphoroamidate. Phosphoryl oxygen of inhibitor is directly coordinated to the catalytic calcium ion | Loligo vulgaris |
Protein Variants | Comment | Organism |
---|---|---|
D229N/N120D | catalytically inactive, no change in the calcium coordinating environment | Loligo vulgaris |
D229N/N175D | catalytically inactive, no change in the calcium coordinating environment | Loligo vulgaris |
E21Q/N120D | catalytically inactive | Loligo vulgaris |
E21Q/N175D | catalytically inactive | Loligo vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O,O-dicyclopentylphosphoroamidate | crystallization data, phosphoryl oxygen of inhibitor is directly coordinated to the catalytic calcium ion | Loligo vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.72 | - |
diisopropyl fluorophosphate | wild-type, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris | |
23.36 | - |
diisopropyl fluorophosphate | wild-type, presence of 1 mM O,O-dicyclopentylphosphoroamidate, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris | |
65.45 | - |
diisopropyl fluorophosphate | wild-type, presence of 3 mM O,O-dicyclopentylphosphoroamidate, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Loligo vulgaris | Q7SIG4 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
diisopropyl fluorophosphate + H2O = diisopropyl phosphate + fluoride | calcium coordinating residue D229, in concert with direct substrate activation by the metal ion, renders the phosphorus atom of the substrate susceptible for attack of water, through generation of a phosphoenzyme intermediate | Loligo vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diisopropyl fluorophosphate + H2O | - |
Loligo vulgaris | diisopropyl phosphate + fluoride | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2091 | - |
diisopropyl fluorophosphate | wild-type, presence of 3 mM O,O-dicyclopentylphosphoroamidate, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris | |
2107 | - |
diisopropyl fluorophosphate | wild-type, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris | |
2111 | - |
diisopropyl fluorophosphate | wild-type, presence of 1 mM O,O-dicyclopentylphosphoroamidate, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
O,O-dicyclopentylphosphoroamidate | wild-type, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris |