Any feedback?
Literature summary for 3.1.8.2 extracted from
- Senescence marker protein-30 is a unique enzyme that hydrolyzes diisopropyl phosphorofluoridate in the liver (2004), FEBS Lett., 570, 57-62.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CdCl2 | 1 mM, stimulates hydrolysis to a lesser extent | Mus musculus |  |
| CdCl2 | 1 mM, stimulates hydrolysis to a lesser extent, 38% activity, compared to MgCl2 | Rattus norvegicus | |
| CoCl2 | 1 mM, stimulates hydrolysis well | Mus musculus | |
| CoCl2 | 1 mM, stimulates hydrolysis well, 102% activity, compared to MgCl2 | Rattus norvegicus | |
| MgCl2 | 1 mM, stimulates hydrolysis well | Mus musculus | |
| MgCl2 | 1 mM, stimulates hydrolysis well, 100% activity | Rattus norvegicus | |
| MnCl2 | 1 mM, stimulates hydrolysis well | Mus musculus | |
| MnCl2 | 1 mM, stimulates hydrolysis well, 100% activity | Rattus norvegicus | |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | the enzyme protects the liver from toxic effects of the substrate diisopropyl phosphorofluoridate | Mus musculus |
| additional information | the enzyme protects the liver from toxic effects of the substrate diisopropyl phosphorofluoridate | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of SMP30 knockout mice showing no activity with diisopropyl phosphofluoridate in livers, the liver of the mutant mice is far more susceptible to cytotoxicity of diisopropyl phosphofluoridate | Mus musculus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | slightly activates the hydrolysis of diisopropyl phosphofluoridate | Rattus norvegicus | |
| Co2+ | fully activates the hydrolysis of diisopropyl phosphofluoridate | Rattus norvegicus | |
| Mg2+ | fully activates the hydrolysis of diisopropyl phosphofluoridate | Rattus norvegicus | |
| Mn2+ | fully activates the hydrolysis of diisopropyl phosphofluoridate | Rattus norvegicus | |
| additional information | no effect by Ca2+ on SMP30, the enzyme does not bind to Ca2+ at all | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Rattus norvegicus |
| 34000 | - |
x * 34000, SMP30, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diisopropyl phosphofluoridate + H2O | Mus musculus | SMP30 is important in diisopropyl phosphofluoridate detoxification | diisopropyl phosphate + fluoride | - |
? | |
| diisopropyl phosphofluoridate + H2O | Rattus norvegicus | SMP30 is important in diisopropyl phosphofluoridate detoxification | diisopropyl phosphate + fluoride | - |
? | |
| additional information | Rattus norvegicus | enzyme expression in liver decreases androgen-independently of aging | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Rattus norvegicus | Q03336 | SMP30, gene rgn; gene rgn | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
- |
Rattus norvegicus |
| SMP30 49fold from liver by anion exchange and hydrophobic interaction chromatography, and gel filtration | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatocyte | - |
Mus musculus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Rattus norvegicus | - |
| liver | SMP30 | Mus musculus | - |
| liver | SMP30 | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.527 | - |
purified SMP30, substrate phenyl acetate | Rattus norvegicus |
| 0.527 | - |
with phenyl acetate as substrate, pH 8.0, 25°C, 1 mM MgCl2 | Rattus norvegicus |
| 0.998 | - |
with diisopropyl phosphorofluoridate as substrate, pH 8.0, 25°C, 1 mM MgCl2 | Rattus norvegicus |
| 1.512 | - |
purified enzyme | Rattus norvegicus |
| 1.512 | - |
purified SMP30, substrate diisopropyl phosphofluoridate | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diisopropyl phosphofluoridate + H2O | - |
Rattus norvegicus | diisopropyl phosphate + fluoride | - |
? | |
| diisopropyl phosphofluoridate + H2O | SMP30 is important in diisopropyl phosphofluoridate detoxification | Mus musculus | diisopropyl phosphate + fluoride | - |
? | |
| diisopropyl phosphofluoridate + H2O | SMP30 is important in diisopropyl phosphofluoridate detoxification | Rattus norvegicus | diisopropyl phosphate + fluoride | - |
? | |
| diisopropyl phosphofluoridate + H2O | substrate of SMP30 | Mus musculus | diisopropyl phosphate + fluoride | - |
? | |
| diisopropyl phosphorofluoridate + H2O | pH 8.0, 25°C, 1 mM MgCl2 | Mus musculus | diisopropyl phosphate + fluoride | - |
? | |
| diisopropyl phosphorofluoridate + H2O | pH 8.0, 25°C, 1 mM MgCl2 | Rattus norvegicus | diisopropyl phosphate + fluoride | - |
? | |
| additional information | enzyme expression in liver decreases androgen-independently of aging | Rattus norvegicus | ? | - |
? | |
| additional information | no activity of SMP30 with paraoxon, dihydrocoumarin, gamma-nonalactone, and delta-dodecanolactone | Mus musculus | ? | - |
? | |
| additional information | no activity of SMP30 with paraoxon, dihydrocoumarin, gamma-nonalactone, and delta-dodecanolactone | Rattus norvegicus | ? | - |
? | |
| additional information | no activity with paraoxon, dihydrocoumarin, gamma-nonalactone and delta-dodecanolactone | Rattus norvegicus | ? | - |
? | |
| phenyl acetate + H2O | - |
Rattus norvegicus | phenol + acetate | - |
? | |
| phenyl acetate + H2O | pH 8.0, 25°C, 1 mM MgCl2 | Rattus norvegicus | phenol + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Rattus norvegicus |
| ? | x * 34000, SMP30, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DFPase | - |
Mus musculus |
| DFPase | - |
Rattus norvegicus |
| More | SMP30 does not belong to the PON family | Mus musculus |
| More | SMP30 does not belong to the PON family | Rattus norvegicus |
| RC | - |
Rattus norvegicus |
| regucalcin | - |
Rattus norvegicus |
| senescence marker protein-30 | - |
Mus musculus |
| senescence marker protein-30 | - |
Rattus norvegicus |
| SMP30 | - |
Mus musculus |
| SMP30 | - |
Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
