Literature summary for 3.1.8.1 extracted from

Falahati-Pour, S.K.; Lotfi, A.S.; Ahmadian, G.; Baghizadeh, A.; Behroozi, R.; Haghighi, F.
High-level extracellular secretion of organophosphorous hydrolase of Flavobacterium sp. in Escherichia coli BL21(DE3)pLysS (2016), Biotechnol. Appl. Biochem., 63, 870-876 .

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of the enzyme in Escherichia coli BL21(DE3)pLysS, subcloning in Escherichia coli strain DH5alpha. To achieve the extracellular secretion of enzyme OPH in Escherichia coli, the complete opd gene along with its native signal peptide is codon-optimized and expressed in Escherichia coli BL21(DE3)pLysS. The secretion of OPH into the extracellular medium is highly affected by culture conditions, e.g. Co2+ decreases the secretion of the enzyme, while IPTG causes an enhancement in extracellular OPH secretion	Flavobacterium sp. MTCC 2495

Cloned (Comment)

Organism

recombinant expression of the enzyme in Escherichia coli BL21(DE3)pLysS, subcloning in Escherichia coli strain DH5alpha. To achieve the extracellular secretion of enzyme OPH in Escherichia coli, the complete opd gene along with its native signal peptide is codon-optimized and expressed in Escherichia coli BL21(DE3)pLysS. The secretion of OPH into the extracellular medium is highly affected by culture conditions, e.g. Co2+ decreases the secretion of the enzyme, while IPTG causes an enhancement in extracellular OPH secretion

Flavobacterium sp. MTCC 2495

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	the premature enzyme contains a signal peptide	Flavobacterium sp. MTCC 2495	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	required	Flavobacterium sp. MTCC 2495
additional information	divalent cations are essential for activity	Flavobacterium sp. MTCC 2495

Organism

Organism	UniProt	Comment	Textmining
Flavobacterium sp. MTCC 2495	Q5UB52	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	the premature enzyme contains a signal peptide that is cleaved off during maturation	Flavobacterium sp. MTCC 2495

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
paraoxon + H2O	-	Flavobacterium sp. MTCC 2495	diethyl phosphate + 4-nitrophenol	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000, premature enzyme with signal peptide, SDS-PAGE, x * 37000, mature enzyme, SDS-PAGE	Flavobacterium sp. MTCC 2495

Synonyms

Synonyms	Comment	Organism
opd	-	Flavobacterium sp. MTCC 2495
OPH	-	Flavobacterium sp. MTCC 2495
organophosphorus hydrolase	-	Flavobacterium sp. MTCC 2495

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Flavobacterium sp. MTCC 2495

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.8	-	assay at	Flavobacterium sp. MTCC 2495