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Literature summary for 3.1.8.1 extracted from

  • Restaino, O.F.; Borzacchiello, M.G.; Scognamiglio, I.; Porzio, E.; Manco, G., Fedele, L., Donatiello, C., De Rosa, M.; Schiraldi, C.
    Boosted large-scale production and purification of a thermostable archaeal phosphotriesterase-like lactonase for organophosphate decontamination (2017), J. Ind. Microbiol. Biotechnol., 44, 363-375.
    View publication on PubMed
Search for more literature for 3.1.8.1

Application

Application Comment Organism
synthesis efficient production of enzyme in Escherichia coli, by designing high cell density cultivations and a membrane-based downstream process. In fed batches, enzyme production is increased by 69fold up to 4660 U/l, using galactose as inducer. the process is scalable from 2.5 up to 150 l. An enzyme recovery of 77% with a purity grade of 80% can be reached Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
W263F mutant is able to convert paraoxon to 4-nitrophenol Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97VT7
-
-
Saccharolobus solfataricus DSM 1617 Q97VT7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.5
-
 pH 8.5, 70°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
paraoxon + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + diethyl phosphate
-
 ?
paraoxon + H2O
-
 Saccharolobus solfataricus DSM 1617 4-nitrophenol + diethyl phosphate
-
 ?

Synonyms

Synonyms Comment Organism
aryldialkylphosphatase
-
 Saccharolobus solfataricus
PHP
-
 Saccharolobus solfataricus
SsoPox
-
 Saccharolobus solfataricus