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Literature summary for 3.1.8.1 extracted from

  • Hiblot, J.; Gotthard, G.; Chabriere, E.; Elias, M.
    Characterisation of the organophosphate hydrolase catalytic activity of SsoPox (2012), Sci. Rep., 2, 779.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.8.1

Activating Compound

Activating Compound Comment Organism Structure
SDS 0.01%, approximately 12.5 times catalytic efficiency enhancement compared to that without detergent Saccharolobus solfataricus
sodium deoxycholate 0.05%, approximately 33times catalytic efficiency enhancement compared to that without detergent Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
fensulfothion
-
 Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 methyl parathion 25°C, pH 9.0 Saccharolobus solfataricus
0.16
-
 malathion 25°C, pH 9.0 Saccharolobus solfataricus
2.14
-
 methyl paraoxon 25°C, pH 9.0 Saccharolobus solfataricus
3.27
-
 paraoxon 70°C, pH 9.0 Saccharolobus solfataricus
24.25
-
 paraoxon 25°C, pH 9.0 Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97VT7
-
-
Saccharolobus solfataricus P2 Q97VT7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malathion + H2O
-
 Saccharolobus solfataricus ?
-
 ?
malathion + H2O
-
 Saccharolobus solfataricus P2 ?
-
 ?
methyl paraoxon + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + dimethyl phosphate
-
 ?
methyl paraoxon + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + dimethyl phosphate
-
 ?
methyl parathion + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + dimethyl thiophosphate
-
 ?
methyl parathion + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + dimethyl thiophosphate
-
 ?
paraoxon + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + diethyl phosphate
-
 ?
paraoxon + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + diethyl phosphate
-
 ?

Synonyms

Synonyms Comment Organism
organophosphate hydrolase
-
 Saccharolobus solfataricus
SSO2522 locus name Saccharolobus solfataricus
SsoPox
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00089
-
 malathion 25°C, pH 9.0 Saccharolobus solfataricus
0.0011
-
 methyl parathion 25°C, pH 9.0 Saccharolobus solfataricus
2.71
-
 methyl paraoxon 25°C, pH 9.0 Saccharolobus solfataricus
3.98
-
 paraoxon 70°C, pH 9.0 Saccharolobus solfataricus
12.59
-
 paraoxon 25°C, pH 9.0 Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.23
-
 fensulfothion 70°C, pH 9.0 Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0056
-
 malathion 25°C, pH 9.0 Saccharolobus solfataricus
0.0091
-
 methyl parathion 25°C, pH 9.0 Saccharolobus solfataricus
0.52
-
 paraoxon 25°C, pH 9.0 Saccharolobus solfataricus
1.22
-
 paraoxon 70°C, pH 9.0 Saccharolobus solfataricus
1.27
-
 methyl paraoxon 25°C, pH 9.0 Saccharolobus solfataricus