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Literature summary for 3.1.8.1 extracted from
- Structural and enzymatic characterization of the lactonase SisLac from Sulfolobus islandicus (2012), PLoS One, 7, e47028.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| SDS | the addition of 0.01% SDS results in an up to a 2.5folds increase in the paraoxonase catalytic efficiency, whereas the addition of 0.1% SDS enhances the efficiency by 25fold | Sulfolobus islandicus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Sulfolobus islandicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E14K | Tm-value for the mutant enzyme is 6°C lower than wild-type Tm-value. The efficiency of methyl paraoxon hydrolysis is similar for wild-type and the mutants, whereas the mutants exhibit higher catalytic efficiency against ethyl-paraoxon than the wild-type. The mutants exhibit a heavily reduced N-acyl-homoserine lactonase-, delta-lactonase- and gamma-lactonase activity | Sulfolobus islandicus |
| E14K/Y34Q | Tm-value for the mutant enzyme is 4°C lower than wild-type Tm-value. The efficiency of methyl paraoxon hydrolysis is similar for wild-type and the mutants, whereas the mutants exhibit higher catalytic efficiency against ethyl-paraoxon than the wild-type. The mutants exhibit a heavily reduced N-acyl-homoserine lactonase-, delta-lactonase- and gamma-lactonase activity | Sulfolobus islandicus |
| Y34Q | Tm-value for the mutant enzyme is 8°C lower than wild-type Tm-value. The efficiency of methyl paraoxon hydrolysis is similar for wild-type and the mutants, whereas the mutants exhibit higher catalytic efficiency against ethyl-paraoxon than the wild-type. The mutants exhibit a heavily reduced N-acyl-homoserine lactonase-, delta-lactonase- and gamma-lactonase activity | Sulfolobus islandicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
paraoxon | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
| 0.05 | - |
methyl paraoxon | 25°C, pH 9, mutant enzyme E14K | Sulfolobus islandicus | |
| 0.132 | - |
paraoxon | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 0.15 | - |
paraoxon | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 0.261 | - |
methyl paraoxon | 25°C, pH 9, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 0.272 | - |
methyl parathion | 25°C, pH 9 | Sulfolobus islandicus | |
| 0.33 | - |
malathion | 25°C, pH 9 | Sulfolobus islandicus | |
| 0.62 | - |
methyl paraoxon | 25°C, pH 9, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 1.1 | - |
paraoxon | 70°C, pH not specified in the publication | Sulfolobus islandicus | |
| 1.74 | - |
methyl paraoxon | 25°C, pH 9, wild-type enzyme | Sulfolobus islandicus | |
| 2 | - |
paraoxon | 25°C, 0.1% SDS, pH not specified in the publication | Sulfolobus islandicus | |
| 4.2 | - |
paraoxon | 25°C, 0.01% SDS, pH not specified in the publication | Sulfolobus islandicus | |
| 5.44 | - |
paraoxon | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
dynamic light scattering | Sulfolobus islandicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus islandicus | C4KKZ9 | - |
- |
| Sulfolobus islandicus M.16.4 | C4KKZ9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus islandicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethyl paraoxon + H2O | - |
Sulfolobus islandicus | ? | - |
? | |
| malathion + H2O | - |
Sulfolobus islandicus | O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate | - |
? | |
| methyl paraoxon + H2O | - |
Sulfolobus islandicus | 4-nitrophenol + dimethyl phosphate | - |
? | |
| methyl parathion + H2O | - |
Sulfolobus islandicus | 4-nitrophenol + dimethyl thiophosphate | - |
? | |
| additional information | no hydrolysis: ethyl parathion. The enzyme also shows activity of 1,4-lactonase (EC 3.1.1.25) and arylesterase (EC 3.1.1.2) | Sulfolobus islandicus | ? | - |
? | |
| paraoxon + H2O | - |
Sulfolobus islandicus | 4-nitrophenol + diethyl phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | at 25°C, dynamic light scattering | Sulfolobus islandicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SisLac | the enzyme also shows activity of 1,4-lactonase (EC 3.1.1.25) and arylesterase (EC 3.1.1.2) | Sulfolobus islandicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 85 | activity increases from 25°C to 85°C | Sulfolobus islandicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life: 84 min | Sulfolobus islandicus |
| 90 | - |
half-life: 8.5 min | Sulfolobus islandicus |
| 94 | - |
Tm-value, mutant enzyme Q34Q | Sulfolobus islandicus |
| 95 | - |
half-life: 3.6 min | Sulfolobus islandicus |
| 96 | - |
Tm-value, mutant enzyme E14K | Sulfolobus islandicus |
| 98 | - |
Tm-value, mutant enzyme E14K/Y34Q | Sulfolobus islandicus |
| 102 | - |
Tm-value, wild-type enzyme | Sulfolobus islandicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
paraoxon | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 0.14 | - |
paraoxon | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
| 0.17 | - |
paraoxon | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 0.3 | - |
methyl paraoxon | 25°C, pH 9, mutant enzyme E14K | Sulfolobus islandicus | |
| 0.42 | - |
methyl paraoxon | 25°C, pH 9, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 0.79 | - |
paraoxon | 70°C, pH not specified in the publication | Sulfolobus islandicus | |
| 0.86 | - |
methyl paraoxon | 25°C, pH 9, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 1.42 | - |
paraoxon | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 2.7 | - |
paraoxon | 25°C, 0.01% SDS, pH not specified in the publication | Sulfolobus islandicus | |
| 6.2 | - |
malathion | 25°C, pH 9 | Sulfolobus islandicus | |
| 7.4 | - |
methyl paraoxon | 25°C, pH 9, wild-type enzyme | Sulfolobus islandicus | |
| 9.7 | - |
methyl parathion | 25°C, pH 9 | Sulfolobus islandicus | |
| 14.31 | - |
paraoxon | 25°C, 0.1% SDS, pH not specified in the publication | Sulfolobus islandicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Sulfolobus islandicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7.0: about 80% of maximal activity, pH 10.0: about 80% of maximal activity | Sulfolobus islandicus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.036 | - |
methyl parathion | 25°C, pH 9 | Sulfolobus islandicus | |
| 0.26 | - |
paraoxon | 25°C, pH not specified in the publication | Sulfolobus islandicus | |
| 0.64 | - |
paraoxon | 25°C, 0.01% SDS, pH not specified in the publication | Sulfolobus islandicus | |
| 0.7 | - |
paraoxon | 70°C, pH not specified in the publication | Sulfolobus islandicus | |
| 0.87 | - |
paraoxon | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 1.3 | - |
paraoxon | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 1.4 | - |
methyl paraoxon | 25°C, pH 9, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 1.6 | - |
methyl paraoxon | 25°C, pH 9, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 1.88 | - |
malathion | 25°C, pH 9 | Sulfolobus islandicus | |
| 4.26 | - |
methyl paraoxon | 25°C, pH 9, wild-type enzyme | Sulfolobus islandicus | |
| 4.5 | - |
paraoxon | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
| 6 | - |
methyl paraoxon | 25°C, pH 9, mutant enzyme E14K | Sulfolobus islandicus | |
| 7.14 | - |
paraoxon | 25°C, 0.1% SDS, pH not specified in the publication | Sulfolobus islandicus | |
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