Cloned (Comment) | Organism |
---|---|
overexpressed in Pseudomonas putida KT2440 | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.36 | - |
paraoxon | pH 8.0, 70°C | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 2.2 Co2+ per active site in the enzyme that is purified from recombinant Pseudomonas putida | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97VT7 | - |
- |
Saccharolobus solfataricus P2 | Q97VT7 | - |
- |
Oxidation Stability | Organism |
---|---|
original color intensity after 23 days of storage in water or 20 mM sodium HEPES buffer (pH 8.0) | Saccharolobus solfataricus |
storage under dry conditions is less favorable for the stability of the enzyme, as the amount of 4-nitrophenolate produced from 1 mM paraoxon is reduced to 60 to 75% of the initial amount after 23 days | Saccharolobus solfataricus |
when the enzyme is immobilized on nanoalumina membranes 25% of the activity is retained upon immobilization. When tested with 1 mM paraoxon, the membranes produced at least 90% of their | Saccharolobus solfataricus |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
paraoxon + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + diethyl phosphate | - |
? | |
paraoxon + H2O | - |
Saccharolobus solfataricus P2 | 4-nitrophenol + diethyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
organophosphate hydrolase | - |
Saccharolobus solfataricus |
paraoxonase | - |
Saccharolobus solfataricus |
SSO2522 | locus name | Saccharolobus solfataricus |
SsoPox | bifunctional enzyme with organophosphate hydrolase and N-acyl homoserine lactonase activities | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.29 | - |
paraoxon | pH 8.0, 70°C | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.97 | - |
paraoxon | pH 8.0, 70°C | Saccharolobus solfataricus |