Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.8.1 extracted from

  • Ng, F.S.; Wright, D.M.; Seah, S.Y.
    Characterization of a phosphotriesterase-like lactonase from Sulfolobus solfataricus and its immobilization for disruption of quorum sensing (2010), Appl. Environ. Microbiol., 77, 1181-1186.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Pseudomonas putida KT2440 Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.36
-
paraoxon pH 8.0, 70°C Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 2.2 Co2+ per active site in the enzyme that is purified from recombinant Pseudomonas putida Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97VT7
-
-
Saccharolobus solfataricus P2 Q97VT7
-
-

Oxidation Stability

Oxidation Stability Organism
original color intensity after 23 days of storage in water or 20 mM sodium HEPES buffer (pH 8.0) Saccharolobus solfataricus
storage under dry conditions is less favorable for the stability of the enzyme, as the amount of 4-nitrophenolate produced from 1 mM paraoxon is reduced to 60 to 75% of the initial amount after 23 days Saccharolobus solfataricus
when the enzyme is immobilized on nanoalumina membranes 25% of the activity is retained upon immobilization. When tested with 1 mM paraoxon, the membranes produced at least 90% of their Saccharolobus solfataricus

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
paraoxon + H2O
-
Saccharolobus solfataricus 4-nitrophenol + diethyl phosphate
-
?
paraoxon + H2O
-
Saccharolobus solfataricus P2 4-nitrophenol + diethyl phosphate
-
?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
organophosphate hydrolase
-
Saccharolobus solfataricus
paraoxonase
-
Saccharolobus solfataricus
SSO2522 locus name Saccharolobus solfataricus
SsoPox bifunctional enzyme with organophosphate hydrolase and N-acyl homoserine lactonase activities Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.29
-
paraoxon pH 8.0, 70°C Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.97
-
paraoxon pH 8.0, 70°C Saccharolobus solfataricus