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Literature summary for 3.1.8.1 extracted from

  • Sayin, D.; Cakir, D.T.; Gencer, N.; Arslan, O.
    Effects of some metals on paraoxonase activity from shark Scyliorhinus canicula (2012), J. Enzyme Inhib. Med. Chem., 27, 595-598.
    View publication on PubMed
Search for more literature for 3.1.8.1

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Scyliorhinus canicula
Cu2+
-
 Scyliorhinus canicula
Hg2+
-
 Scyliorhinus canicula
Ni2+
-
 Scyliorhinus canicula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 x * 66000, SDS-PAGE Scyliorhinus canicula

Organism

Organism UniProt Comment Textmining
Scyliorhinus canicula
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sepharose-4Bl-tyrosine-1-naphthylamine column chromatography Scyliorhinus canicula

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Scyliorhinus canicula
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.344
-
 crude enzyme, pH 8.0, 37°C Scyliorhinus canicula
12.84
-
 after 37.3fold purification, pH 8.0, 37°C Scyliorhinus canicula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
paraoxon + H2O
-
 Scyliorhinus canicula 4-nitrophenol + diethyl phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Scyliorhinus canicula

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.29
-
 pH 8.0, 37°C Scyliorhinus canicula Cu2+
0.49
-
 pH 8.0, 37°C Scyliorhinus canicula Hg2+
0.73
-
 pH 8.0, 37°C Scyliorhinus canicula Cd2+
2
-
 pH 8.0, 37°C Scyliorhinus canicula Ni2+