Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL-21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F132G | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
G60A | the mutant shows increased catalytic efficiency with sarin and soman compared to the wild type enzyme | Homo sapiens |
H254Q/H257F | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
H257Y/L303T | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
I106A/F132A/H257Y | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
I106A/H257Y/S308A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
I106G | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
I106G/F132G/H257Y | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
I106G/H257Y | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
S308G | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the native enzyme contains two Zn2+ ions, and these metal ions can be substituted with Cd2+, Co2+, Ni2+, or Mn2+ without a loss of catalytic activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Ultrogel AcA 54 gel filtration, and DEAE-Sephadex A-25 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | - |
? | |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | - |
? | |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (R)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + 4-acetylphenol + (2S)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | - |
? | |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | - |
? | |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + 2-methylpropyl (R)-methylphosphonate | - |
? | |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + 2-methylpropyl (S)-methylphosphonate | - |
? | |
4-acetylphenyl cyclohexyl (R)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + cyclohexyl (R)-methylphosphonate | - |
? | |
4-acetylphenyl cyclohexyl (S)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + cyclohexyl (S)-methylphosphonate | - |
? | |
4-acetylphenyl ethyl (R)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + ethyl (R)-methylphosphonate | - |
? | |
4-acetylphenyl ethyl (S)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + ethyl (S)-methylphosphonate | - |
? | |
4-acetylphenyl propan-2-yl (R)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + propan-2-yl (R)-methylphosphonate | - |
? | |
4-acetylphenyl propan-2-yl (S)-methylphosphonate + H2O | - |
Homo sapiens | 4-acetylphenol + propan-2-yl (S)-methylphosphonate | - |
? | |
cyclosarin + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | the stereochemical preference of the wild-type enzyme with chemical warfare agents is for the RP enantiomers | Homo sapiens | ? | - |
? | |
paraoxon + H2O | best substrate | Homo sapiens | 4-nitrophenol + diethyl phosphate | - |
? | |
sarin + H2O | - |
Homo sapiens | ? | - |
? | |
soman + H2O | - |
Homo sapiens | ? | - |
? | |
tabun + H2O | - |
Homo sapiens | ? | - |
? | |
VR + H2O | - |
Homo sapiens | ? | - |
? | |
VX + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
phosphotriesterase | - |
Homo sapiens |
PTE | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.12 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.14 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.21 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.41 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.45 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.62 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.81 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
1.5 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
2 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
2.1 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
2.2 | - |
soman | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
2.2 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
2.6 | - |
soman | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
2.9 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.4 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
5.1 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
5.9 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
7.3 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
7.4 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
8.5 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
9.1 | - |
soman | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
12 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
14 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
15 | - |
sarin | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
15 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
18 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
19 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
20 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
22 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
25 | - |
soman | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
37 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
39 | - |
sarin | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
40 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
50 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
51 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
89 | - |
soman | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
90 | - |
sarin | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
92 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
93 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
93 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
100 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
100 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
110 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
110 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
110 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
120 | - |
sarin | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
120 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
130 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
150 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
150 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
190 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
250 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
290 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
300 | - |
sarin | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
370 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
410 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
670 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00094 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.0011 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.003 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.0032 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.0067 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.016 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.021 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.058 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.099 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.1 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.13 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.2 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.22 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.25 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.25 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.49 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
0.59 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
1.3 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
1.5 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
1.5 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
1.7 | - |
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
1.8 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
1.9 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
2.3 | - |
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
2.4 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
2.5 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
3.1 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
5.8 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
5.9 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
6.2 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
9.5 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
13 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
16 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
21 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
27 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
28 | - |
4-acetylphenyl cyclohexyl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
28 | - |
4-acetylphenyl cyclohexyl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
34 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
73 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
75 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
110 | - |
4-acetylphenyl propan-2-yl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
110 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
130 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
150 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
160 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
180 | - |
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
180 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
220 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
490 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
520 | - |
4-acetylphenyl ethyl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
520 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
580 | - |
4-acetylphenyl propan-2-yl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
760 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
850 | - |
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens | |
1200 | - |
4-acetylphenyl ethyl (S)-methylphosphonate | wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C | Homo sapiens |