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Literature summary for 3.1.8.1 extracted from

  • Otto, T.C.; Kasten, S.A.; Kovaleva, E.; Liu, Z.; Buchman, G.; Tolosa, M.; Davis, D.; Smith, J.R.; Balcerzak, R.; Lenz, D.E.; Cerasoli, D.M.
    Purification and characterization of functional human paraoxonase-1 expressed in Trichoplusia ni larvae (2010), Chem. Biol. Interact., 187, 388-392.
    View publication on PubMed
Search for more literature for 3.1.8.1

Application

Application Comment Organism
analysis PON1 has the potential to be used as a catalytic bioscavenger of nerve agents. Insect production of PON1 may provide a source for both in vitro enzymatic and crystallographic studies and in vivo stability and anti-nerve agent efficacy testing Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed from Trichoplusia ni larvae Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 O-isobutyl-S-[2-(diethylamino)ethyl]methylphosphonothioic acid in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4 Homo sapiens
1.5
-
 paraoxon in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 x * 41000, glycosylated PON1, SDS-PAGE or mass spectrometry, x * 42000, glycosylated PON1, SDS-PAGE or mass spectrometry, x * 44000, glycosylated PON1, SDS-PAGE or mass spectrometry Homo sapiens
42000
-
 x * 41000, glycosylated PON1, SDS-PAGE or mass spectrometry, x * 42000, glycosylated PON1, SDS-PAGE or mass spectrometry, x * 44000, glycosylated PON1, SDS-PAGE or mass spectrometry Homo sapiens
44000
-
 x * 41000, glycosylated PON1, SDS-PAGE or mass spectrometry, x * 42000, glycosylated PON1, SDS-PAGE or mass spectrometry, x * 44000, glycosylated PON1, SDS-PAGE or mass spectrometry Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by immobilized metal-ion affinity chromatography Homo sapiens

Storage Stability

Storage Stability Organism
4°C, 2 weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclosarin + H2O
-
 Homo sapiens methyl-phosphonic acid monofluoride + cyclohexanol
-
 ?
additional information is resistant to chlorpyrifos Homo sapiens ?
-
 ?
O-ethyl-S-[2-(diisopropylamino)ethyl]-methylphosphonothioic acid + H2O hydrolysis is exclusively preferential for the P+ isomer. Glycosylation state of PON1 does not affect substrate stereoselectivity Homo sapiens S-[2-(diisopropylamino)ethyl]-methylphosphonothioic acid + ethanol
-
 ?
O-isobutyl-S-[2-(diethylamino)ethyl]methylphosphonothioic acid + H2O hydrolysis is exclusively preferential for the P+ isomer. Glycosylation state of PON1 does not affect substrate stereoselectivity Homo sapiens ?
-
 ?
paraoxon + H2O
-
 Homo sapiens 4-nitrophenol + diethyl phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, glycosylated PON1, SDS-PAGE or mass spectrometry, x * 42000, glycosylated PON1, SDS-PAGE or mass spectrometry, x * 44000, glycosylated PON1, SDS-PAGE or mass spectrometry Homo sapiens

Synonyms

Synonyms Comment Organism
paraoxonase-1
-
 Homo sapiens
PON1
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 O-isobutyl-S-[2-(diethylamino)ethyl]methylphosphonothioic acid in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4 Homo sapiens
0.45
-
 paraoxon in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4 Homo sapiens

General Information

General Information Comment Organism
physiological function caterpillar of the Trichoplusia ni moth can be used as an expression system to produce large quantities of functional recombinant PON1 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00083
-
 cyclosarin in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4 Homo sapiens
0.2
-
 O-isobutyl-S-[2-(diethylamino)ethyl]methylphosphonothioic acid in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4 Homo sapiens
0.2
-
 O-ethyl-S-[2-(diisopropylamino)ethyl]-methylphosphonothioic acid in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4 Homo sapiens
0.3
-
 paraoxon in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4 Homo sapiens