Application | Comment | Organism |
---|---|---|
drug development | PON1 variants with catalytic efficacy that will qualify them as potential drug candidate for prophylactic treatment of nerve agent intoxication | synthetic construct |
Cloned (Comment) | Organism |
---|---|
- |
synthetic construct |
Protein Variants | Comment | Organism |
---|---|---|
H115W | as the wild-type, preference for the three RP isomers of the O-alkyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin analogs, regardless of the size of the alkyl group | synthetic construct |
additional information | variant 3B3 hydrolyzes almost exclusively the RP isomer whereas variant 3D8 is capable of hydrolyzing both RP and SP enantiomers. Variant 3B3 displays even greater stereo-specificity towards the RP enantiomers as the wild-type and mutant H115W | synthetic construct |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
synthetic construct | - |
variant G3C9 of the gene shuffling products of four mammalian PON1s | - |
Purification (Comment) | Organism |
---|---|
- |
synthetic construct |
Storage Stability | Organism |
---|---|
4°C, 2 weeks | synthetic construct |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RP-O-ethyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin + H2O | - |
synthetic construct | ? | - |
? | |
RP-O-n-propyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin + H2O | - |
synthetic construct | ? | - |
? | |
SP-O-ethyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin + H2O | - |
synthetic construct | ? | - |
? | |
SP-O-n-propyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin + H2O | - |
synthetic construct | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
paraoxonase | - |
synthetic construct |
PON1 | - |
synthetic construct |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
SP-O-ethyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | wild-type, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
0.167 | - |
RP-O-n-propyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | mutant H115W, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
0.5 | - |
SP-O-ethyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | mutant H115W, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
0.55 | - |
RP-O-ethyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | mutant H115W, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
1.83 | - |
SP-O-n-propyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | wild-type, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
4.95 | - |
RP-O-n-propyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | wild-type, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
6.467 | - |
SP-O-n-propyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | mutant H115W, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
11.72 | - |
RP-O-ethyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | wild-type, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.05 | - |
SP-O-ethyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | wild-type, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
6.7 | - |
SP-O-ethyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | mutant H115W, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
13.6 | - |
SP-O-n-propyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | wild-type, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
25 | - |
SP-O-n-propyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | mutant H115W, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
617 | - |
RP-O-ethyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | wild-type, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
767 | - |
RP-O-ethyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | mutant H115W, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
883 | - |
RP-O-n-propyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | mutant H115W, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct | |
933 | - |
RP-O-n-propyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin | wild-type, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C | synthetic construct |