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Literature summary for 3.1.8.1 extracted from
- A thermostable phosphotriesterase from the archaeon Sulfolobus solfataricus: cloning, overexpression and properties (2005), Extremophiles, 9, 297-305.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain HB101 and BL21(DE3) | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | inactivation | Saccharolobus solfataricus |  |
| fensulfothion | - |
Saccharolobus solfataricus | |
| o-phenanthroline | inactivation | Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | first order kinetics | Saccharolobus solfataricus | |
| 0.06 | - |
paraoxon | pH 8.0, 70°C, recombinant enzyme | Saccharolobus solfataricus | |
| 0.205 | - |
methyl paraoxon | pH 8.0, 70°C, recombinant enzyme | Saccharolobus solfataricus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | the enzyme depends on divalent metal ions with Co2+, Mg2+, and Ni2+ being the most effective | Saccharolobus solfataricus | |
| Mg2+ | the enzyme depends on divalent metal ions with Co2+, Mg2+, and Ni2+ being the most effective | Saccharolobus solfataricus | |
| Ni2+ | the enzyme depends on divalent metal ions with Co2+, Mg2+, and Ni2+ being the most effective | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE, x * 35565, sequence calculation, the enzyme is in a monomer-dimer equilibrium | Saccharolobus solfataricus |
| 35565 | - |
x * 34000, SDS-PAGE, x * 35565, sequence calculation, the enzyme is in a monomer-dimer equilibrium | Saccharolobus solfataricus |
| 48000 | 50000 | recombinant enzyme, gel filtration | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
| Saccharolobus solfataricus MT-4 / DSM 5833 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli by ultracentrifugation, anion exchange chromatography, and hydrophobic interaction chromatography | Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00042 | - |
purified recombinant enzyme, substrate paraoxon | Saccharolobus solfataricus |
| 0.0022 | - |
purified recombinant enzyme, substrate methyl paraoxon | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl paraoxon + H2O | the enzyme from Sulfolobus solfataricus has a low paraoxonase activity | Saccharolobus solfataricus | 4-nitrophenol + dimethylphosphate | - |
? | |
| methyl paraoxon + H2O | the enzyme from Sulfolobus solfataricus has a low paraoxonase activity | Saccharolobus solfataricus MT-4 / DSM 5833 | 4-nitrophenol + dimethylphosphate | - |
? | |
| additional information | substrate specificity with organophosphorous compounds, overview | Saccharolobus solfataricus | ? | - |
? | |
| additional information | substrate specificity with organophosphorous compounds, overview | Saccharolobus solfataricus MT-4 / DSM 5833 | ? | - |
? | |
| paraoxon + H2O | the enzyme from Sulfolobus solfataricus has a low paraoxonase activity | Saccharolobus solfataricus | 4-nitrophenol + diethyl phosphate | - |
? | |
| paraoxon + H2O | the enzyme from Sulfolobus solfataricus has a low paraoxonase activity | Saccharolobus solfataricus MT-4 / DSM 5833 | 4-nitrophenol + diethyl phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer or dimer | x * 34000, SDS-PAGE, x * 35565, sequence calculation, the enzyme is in a monomer-dimer equilibrium | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphotriesterase | - |
Saccharolobus solfataricus |
| PTE | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
and above | Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 95 | - |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 85 | purified recombinant enzyme, 4 h, completely stable | Saccharolobus solfataricus |
| 95 | - |
purified recombinant enzyme, half-life is 4 h | Saccharolobus solfataricus |
| 100 | - |
purified recombinant enzyme, half-life is 90 min | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
paraoxon | pH 8.0, 70°C, recombinant enzyme | Saccharolobus solfataricus | |
| 1.3 | - |
methyl paraoxon | pH 8.0, 70°C, recombinant enzyme | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | broad optimum | Saccharolobus solfataricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | - |
Saccharolobus solfataricus |
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