Literature summary for 3.1.8.1 extracted from

Jackson, C.J.; Carr, P.D.; Kim, H.K.; Liu, J.W.; Herrald, P.; Mitic, N.; Schenk, G.; Smith, C.A.; Ollis, D.L.
Anomalous scattering analysis of Agrobacterium radiobacter phosphotriesterase: the prominent role of iron in the heterobinuclear active site (2006), Biochem. J., 397, 501-508.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene opdA, expression in Escherichia coli strain DH5alpha, growth in media supplemented with different divalent metal ions, overview	Agrobacterium tumefaciens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme complexed with Co2+, hanging drop vapour diffusion method, 0.005 ml of protein solution containing 6.4 mg/ml protein in 50 mM Hepes, pH 7.0, 150 mM NaCl, and 1 mM CoCl2, are mixed with 0.005 ml of reservoir solution that consists of 20% w/v PEG 3350 and 0.2 M sodium nitrate, X-ray diffraction structure determination and analysis at 1.9 A resolution	Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.36	-	methyl parathion	pH 9.1, 37°C, recombinant enzyme in presence of Co2+	Agrobacterium tumefaciens
0.41	-	methyl parathion	pH 9.1, 37°C, recombinant enzyme in absence of Co2+	Agrobacterium tumefaciens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	activates the enzyme, the enzyme is a natively heterobinuclear iron-zinc metalloprotein, overview, Fe2+ is essential, but can be replaced by Co2+ in supplemented medium for growth of Escherichia coli cells recombinantly expressing the enzyme, Co2+ addition leads to higher activity compared to Fe2+	Agrobacterium tumefaciens
Fe2+	the enzyme is a natively heterobinuclear iron-zinc metalloprotein, overview, Fe2+ is essential, but can be replaced by Co2+ in supplemented medium for growth of Escherichia coli cells recombinantly expressing the enzyme	Agrobacterium tumefaciens
additional information	metal analysis of wild-type and recombinant enzymes, overview	Agrobacterium tumefaciens
Zn2+	the enzyme is a natively heterobinuclear iron-zinc metalloprotein, overview	Agrobacterium tumefaciens

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium tumefaciens	Q93LD7	gene opdA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant OpdA from Escherichia coli strain DH5alpha by anion exchange chromatography, dialysis, sulphopropyl affinity chromatography, and again dialysis	Agrobacterium tumefaciens

Reaction

Reaction	Comment	Organism	Reaction ID
an aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol	catalytic mechanism	Agrobacterium tumefaciens	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
34	-	recombinant enzyme in absence of Co2+	Agrobacterium tumefaciens
168	-	recombinant enzyme in presence of Co2+	Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methyl parathion + H2O	i.e. O,O-dimethyl O-4-nitrophenyl phosphorothioate	Agrobacterium tumefaciens	dimethyl thiophosphate + 4-nitrophenol	-	?

Synonyms

Synonyms	Comment	Organism
OpdA	-	Agrobacterium tumefaciens
phosphotriesterase	-	Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.1	-	assay at	Agrobacterium tumefaciens

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Release 2023.1 (January 2023)