Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.8.1 extracted from

  • Elias, M.; Dupuy, J.; Merone, L.; Lecomte, C.; Rossi, M.; Masson, P.; Manco, G.; Chabriere, E.
    Crystallization and preliminary X-ray diffraction analysis of the hyperthermophilic Sulfolobus solfataricus phosphotriesterase (2007), Acta Crystallogr. Sect. F, 63, 553-555.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging-drop vapour-diffusion technique, 5.8 mg/ml protein in 20 mM HEPES, pH 8.5, 0.2 mM CoCl2 and 0.2 M NaCl, 0.001-0.002 ml of protein and reservoir solutions are mixed and equilibrated against 0.8 ml reservoir solution containing 15-18% w/v PEG 8000 and 50 mM Tris-HCl buffer pH 8.0, 1 week at 4°C, X-ray diffraction structure determination and analysis at 2.54 A resolution Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a organophosphate-degrading enzyme Saccharolobus solfataricus ?
-
?
additional information a organophosphate-degrading enzyme Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
?

Synonyms

Synonyms Comment Organism
phosphotriesterase
-
Saccharolobus solfataricus
PTE
-
Saccharolobus solfataricus
SsoPox
-
Saccharolobus solfataricus