Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging-drop vapour-diffusion technique, 5.8 mg/ml protein in 20 mM HEPES, pH 8.5, 0.2 mM CoCl2 and 0.2 M NaCl, 0.001-0.002 ml of protein and reservoir solutions are mixed and equilibrated against 0.8 ml reservoir solution containing 15-18% w/v PEG 8000 and 50 mM Tris-HCl buffer pH 8.0, 1 week at 4°C, X-ray diffraction structure determination and analysis at 2.54 A resolution | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT-4 / DSM 5833 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | a organophosphate-degrading enzyme | Saccharolobus solfataricus | ? | - |
? | |
additional information | a organophosphate-degrading enzyme | Saccharolobus solfataricus MT-4 / DSM 5833 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphotriesterase | - |
Saccharolobus solfataricus |
PTE | - |
Saccharolobus solfataricus |
SsoPox | - |
Saccharolobus solfataricus |