Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.8.1 extracted from

  • Fu, G.; Cui, Z.; Huang, T.; Li, S.
    Expression, purification, and characterization of a novel methyl parathion hydrolase (2004), Protein Expr. Purif., 36, 170-176.
    View publication on PubMed
Search for more literature for 3.1.8.1

Cloned(Commentary)

Cloned (Comment) Organism
gene mpd, subcloning in Escherichia coli strain DH5alpha, expression of functional His-tagged enzyme in Escherichia coli strain BL21(DE3) improved by elimination of the signal peptide Plesiomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33700
-
 recombinant His-tagged mature enzyme, gel filtration Plesiomonas sp.
35000
-
 1 * 35000, recombinant His-tagged mature enzyme, SDS-PAGE Plesiomonas sp.

Organism

Organism UniProt Comment Textmining
Plesiomonas sp. Q9ALW1 gene mpd
-
Plesiomonas sp. M6 Q9ALW1 gene mpd
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme precursor contains a 36 amino acid N-terminal signal peptide Plesiomonas sp.

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 23.4fold from Escherichia coli by nickel affinity chromatography under non-dneturating and under denaturating conditions, overview Plesiomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53.8
-
 purified recombinant His-tagged mature enzyme Plesiomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl parathion + H2O
-
 Plesiomonas sp. 4-nitrophenol + dimethyl thiophosphate
-
 ?
methyl parathion + H2O
-
 Plesiomonas sp. M6 4-nitrophenol + dimethyl thiophosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 35000, recombinant His-tagged mature enzyme, SDS-PAGE Plesiomonas sp.
More the enzyme precursor contains a 36 amino acid N-terminal signal peptide which is cleaved off to give the mature, active enzyme Plesiomonas sp.

Synonyms

Synonyms Comment Organism
methyl parathion hydrolase
-
 Plesiomonas sp.
Mph
-
 Plesiomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Plesiomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Plesiomonas sp.