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Literature summary for 3.1.8.1 extracted from

  • Roodveldt, C.; Tawfik, D.S.
    Directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression in Escherichia coli results in stabilization of the metal-free state (2005), Protein Eng. Des. Sel., 18, 51-58.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression in Escherichia coli results in stabilization of the metal-free state Brevundimonas diminuta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
paraoxon wild-type enzyme Brevundimonas diminuta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
x * 82000, SDS-PAGE Brevundimonas diminuta

Organism

Organism UniProt Comment Textmining
Brevundimonas diminuta P0A434
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Brevundimonas diminuta

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
paraoxon + H2O
-
Brevundimonas diminuta 4-nitrophenol + diethyl phosphate
-
?

Subunits

Subunits Comment Organism
? x * 82000, SDS-PAGE Brevundimonas diminuta

Synonyms

Synonyms Comment Organism
phosphotriesterase
-
Brevundimonas diminuta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
780
-
paraoxon pH 8.5, recombinant wild-type enzyme-MBP fusion protein Brevundimonas diminuta
1280
-
paraoxon pH 8.5, recombinant genetic variants 1.5E-MBP fusion protein Brevundimonas diminuta
1430
-
paraoxon pH 8.5, recombinant genetic variants 1.6F-MBP fusion protein Brevundimonas diminuta
2150
-
paraoxon pH 8.5, recombinant genetic variants 2.S5-MBP fusion protein Brevundimonas diminuta
2280
-
paraoxon wild-type enzyme Brevundimonas diminuta
2280
-
paraoxon pH 9.0, recombinant wild-type enzyme Brevundimonas diminuta