Cloned (Comment) | Organism |
---|---|
directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression in Escherichia coli results in stabilization of the metal-free state | Brevundimonas diminuta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
paraoxon | wild-type enzyme | Brevundimonas diminuta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
x * 82000, SDS-PAGE | Brevundimonas diminuta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevundimonas diminuta | P0A434 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Brevundimonas diminuta |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
paraoxon + H2O | - |
Brevundimonas diminuta | 4-nitrophenol + diethyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 82000, SDS-PAGE | Brevundimonas diminuta |
Synonyms | Comment | Organism |
---|---|---|
phosphotriesterase | - |
Brevundimonas diminuta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
780 | - |
paraoxon | pH 8.5, recombinant wild-type enzyme-MBP fusion protein | Brevundimonas diminuta | |
1280 | - |
paraoxon | pH 8.5, recombinant genetic variants 1.5E-MBP fusion protein | Brevundimonas diminuta | |
1430 | - |
paraoxon | pH 8.5, recombinant genetic variants 1.6F-MBP fusion protein | Brevundimonas diminuta | |
2150 | - |
paraoxon | pH 8.5, recombinant genetic variants 2.S5-MBP fusion protein | Brevundimonas diminuta | |
2280 | - |
paraoxon | wild-type enzyme | Brevundimonas diminuta | |
2280 | - |
paraoxon | pH 9.0, recombinant wild-type enzyme | Brevundimonas diminuta |