Cloned (Comment) | Organism |
---|---|
the recombinant enzyme is expressed in Trichoplusia ni High Five insect cells | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, recombinant enzyme | Homo sapiens |
40000 | - |
2 * 40000, recombinant enzyme, SDS-PAGE | Homo sapiens |
91900 | - |
serum PON1, nondenaturing PAGE | Homo sapiens |
94900 | - |
recombinant enzyme, nondenaturing PAGE | Homo sapiens |
95600 | - |
recombinant PON1, nondenaturing PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27169 | - |
- |
Homo sapiens | Q15166 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood serum | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.205 | - |
pH 8.5, 25°C, substrate: paraoxon, purified recombinant enzyme | Homo sapiens |
1.94 | - |
pH 8.5, 25°C, substrate: paraoxon, purified recombinant enzyme | Homo sapiens |
40.9 | - |
pH 8.5, 25°C, substrate: chlorpyrifos oxon, purified recombinant enzyme | Homo sapiens |
113 | - |
pH 8.5, 25°C, substrate: diazoxon, purified recombinant enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, 6 months, less than 15% loss of activity | Homo sapiens |
4°C, purified recombinant PON1 preparations typically lose about 20% of the arylesterase activity within the first month after purification, compared with less than 10% loss for the enzyme purified from serum. There is no further significant loss of the PON1 activity afterward, suggesting the existence of two pools in the purified PON1, a labile one and a stable one | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorpyrifos oxon + H2O | - |
Homo sapiens | 3,5,6-trichloro-pyridin-2-ol + diethyl phosphate | - |
? | |
diazoxon + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | no activity with chlorpyrifos oxon and diazoxon | Homo sapiens | ? | - |
? | |
paraoxon + H2O | - |
Homo sapiens | 4-nitrophenol + diethyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, recombinant enzyme, SDS-PAGE | Homo sapiens |
dimer | 2 * 39000, recombinant enzyme | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PON1 | - |
Homo sapiens |
PON3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Homo sapiens |