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Literature summary for 3.1.8.1 extracted from
- The histidine 115-histidine 134 dyad mediates the lactonase activity of mammalian serum paraoxonases (2006), J. Biol. Chem., 281, 7649-7656.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H115A | activity with paraoxon is 167% of wild-type activity, activity with 7-diethylphosphoro-3-cyanocoumarin is 119% of wild-type activity | Homo sapiens |
| H115Q | activity with paraoxon is 32% of wild-type activity, activity with 7-diethylphosphoro-3-cyanocoumarin is 25% of wild-type activity | Homo sapiens |
| H115W | activity with paraoxon is 195% of wild-type activity, activity with 7-diethylphosphoro-3-cyanocoumarin is 234% of wild-type activity | Homo sapiens |
| H134Q | activity with paraoxon is 602% of wild-type activity, activity with 7-diethylphosphoro-3-cyanocoumarin is 38% of wild-type activity | Homo sapiens |
| H184Q | activity with paraoxon is 8.9% of wild-type activity, activity with 7-diethylphosphoro-3-cyanocoumarin is 7.9% of wild-type activity | Homo sapiens |
| H184T | activity with paraoxon is 9.3% of wild-type activity, activity with 7-diethylphosphoro-3-cyanocoumarin is 2.7% of wild-type activity | Homo sapiens |
| H285Q | activity with paraoxon is 13% of wild-type activity, activity with 7-diethylphosphoro-3-cyanocoumarin is 4.3% of wild-type activity | Homo sapiens |
| H285S | activity with paraoxon is 53% of wild-type activity, activity with 7-diethylphosphoro-3-cyanocoumarin is 25% of wild-type activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
paraoxon | pH 8.0, mutant enzyme H134Q | Homo sapiens |  |
| 0.86 | - |
paraoxon | pH 8.0, wild-type enzyme | Homo sapiens | |
| 1.18 | - |
paraoxon | pH 8.0, mutant enzyme H115Q/H134Q | Homo sapiens | |
| 1.35 | - |
paraoxon | pH 8.0, mutant enzyme H115Q | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | high density lipoprotein-associated enzyme | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | Asp269 and Glu53 participate in the ligation of catalytic Ca2+ | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P27169 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-(thiobutyryl)butyrolactone + H2O | - |
Homo sapiens | ? | - |
? | |
| 7-diethylphosphoro-3-cyanocoumarin + H2O | - |
Homo sapiens | 3-cyanocoumarin + diethyl phosphate | - |
? | |
| paraoxon + H2O | - |
Homo sapiens | 4-nitrophenol + diethyl phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PON1 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| 1 | - |
paraoxon | pH 8.0, mutant enzyme H115Q | Homo sapiens | |
| 1.09 | - |
paraoxon | pH 8.0, recombinant wild-type PON1 genetic variant G2E6 | Homo sapiens | |
| 4.2 | - |
paraoxon | pH 8.0, wild-type enzyme | Homo sapiens | |
| 4.7 | - |
paraoxon | pH 8.0, mutant enzyme H115Q/H134Q | Homo sapiens | |
| 12.7 | - |
paraoxon | pH 8.0, mutant enzyme H134Q | Homo sapiens | |
| 166 | - |
5-(thiobutyryl)butyrolactone | pH 8.0, recombinant wild-type PON1 genetic variant G2E6 | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH-rate profile of PON1 with paraoxon | Homo sapiens |
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Release 2023.1 (January 2023)
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