Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 13% activation at 1 mM | Purpureocillium lilacinum | |
dithioerythritol | 20% activation at 1 mM | Purpureocillium lilacinum | |
DTT | 28% activation at 1 mM | Purpureocillium lilacinum | |
additional information | poor activation by glutathione at 1 mM | Purpureocillium lilacinum | |
SDS | 18% activation at 0.5 mM | Purpureocillium lilacinum | |
Triton X-100 | 31% activation at 0.5 mM | Purpureocillium lilacinum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 7% inhibition at 1 mM | Purpureocillium lilacinum | |
4-chloromercuribenzoate | complete inhibition at 0.1 mM | Purpureocillium lilacinum | |
Fe3+ | 88% inhibition at 1 mM | Purpureocillium lilacinum | |
Hg2+ | 94% inhibition at 1 mM | Purpureocillium lilacinum | |
iodoacetic acid | 92% inhibition at 0.1 mM | Purpureocillium lilacinum | |
additional information | Zn2+, Mn2+, EDTA, and Mg2+ are poor inhibitors at 1 mM | Purpureocillium lilacinum | |
NEM | complete inhibition at 0.1 mM | Purpureocillium lilacinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Purpureocillium lilacinum | |
0.033 | - |
paraoxon | pH 7.2, 25°C | Purpureocillium lilacinum | |
0.052 | - |
methyl parathion | pH 7.2, 25°C | Purpureocillium lilacinum | |
0.058 | - |
parathion | pH 7.2, 25°C | Purpureocillium lilacinum | |
0.071 | - |
Coumaphos | pH 7.2, 25°C | Purpureocillium lilacinum | |
0.187 | - |
phosmet | pH 7.2, 25°C | Purpureocillium lilacinum | |
0.236 | - |
demeton-S | pH 7.2, 25°C | Purpureocillium lilacinum | |
0.351 | - |
malathion | pH 7.2, 25°C | Purpureocillium lilacinum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Purpureocillium lilacinum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Purpureocillium lilacinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Purpureocillium lilacinum | - |
- |
- |
Purpureocillium lilacinum BP303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native soluble enzyme 27.3fold by ultracentrifugation, ammonium sulfate precipitation, gel filtration, and ion exchange chromatography to homogeneity | Purpureocillium lilacinum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Purpureocillium lilacinum |
76.3 | - |
purified enzyme, substrate 4-nitrophenyl phosphate at pH 7.0 and 25°C | Purpureocillium lilacinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Purpureocillium lilacinum | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Purpureocillium lilacinum BP303 | 4-nitrophenol + phosphate | - |
? | |
coumaphos + H2O | - |
Purpureocillium lilacinum | 3-chloro-4-methylumbelliferone + diethyl thiophosphate | - |
? | |
demeton-S + H2O | - |
Purpureocillium lilacinum | 2-ethylsulfanyl-ethanethiol + diethyl phosphate | - |
? | |
malathion + H2O | - |
Purpureocillium lilacinum | O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate | - |
? | |
methyl parathion + H2O | - |
Purpureocillium lilacinum | 4-nitrophenol + dimethyl thiophosphate | - |
? | |
methyl parathion + H2O | - |
Purpureocillium lilacinum BP303 | 4-nitrophenol + dimethyl thiophosphate | - |
? | |
additional information | substrate specificity, overview | Purpureocillium lilacinum | ? | - |
? | |
additional information | substrate specificity, overview | Purpureocillium lilacinum BP303 | ? | - |
? | |
paraoxon + H2O | - |
Purpureocillium lilacinum | 4-nitrophenol + diethyl phosphate | - |
? | |
paraoxon + H2O | - |
Purpureocillium lilacinum BP303 | 4-nitrophenol + diethyl phosphate | - |
? | |
parathion + H2O | - |
Purpureocillium lilacinum | 4-nitrophenol + diethyl thiophosphate | - |
? | |
parathion + H2O | - |
Purpureocillium lilacinum BP303 | 4-nitrophenol + diethyl thiophosphate | - |
? | |
phosmet + H2O | - |
Purpureocillium lilacinum | N-hydroxymethyl-phthalimide + O,O-dimethyl dithiophosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Purpureocillium lilacinum |
Synonyms | Comment | Organism |
---|---|---|
OPH | - |
Purpureocillium lilacinum |
organophosphorus pesticide hydrolase | - |
Purpureocillium lilacinum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Purpureocillium lilacinum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 65 | - |
Purpureocillium lilacinum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
3 min, purified enzyme, stable up to | Purpureocillium lilacinum |
50 | - |
3 min, purified enzyme, about 50% remaining activity | Purpureocillium lilacinum |
60 | - |
3 min, purified enzyme, 10% remaining activity | Purpureocillium lilacinum |
70 | - |
3 min, purified enzyme, inactivation | Purpureocillium lilacinum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | - |
malathion | pH 7.2, 25°C | Purpureocillium lilacinum | |
650 | - |
demeton-S | pH 7.2, 25°C | Purpureocillium lilacinum | |
771 | - |
phosmet | pH 7.2, 25°C | Purpureocillium lilacinum | |
949 | - |
Coumaphos | pH 7.2, 25°C | Purpureocillium lilacinum | |
1098 | - |
parathion | pH 7.2, 25°C | Purpureocillium lilacinum | |
1152 | - |
methyl parathion | pH 7.2, 25°C | Purpureocillium lilacinum | |
1336 | - |
paraoxon | pH 7.2, 25°C | Purpureocillium lilacinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Purpureocillium lilacinum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Purpureocillium lilacinum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Purpureocillium lilacinum | isoelectric focusing | - |
4.8 |