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Literature summary for 3.1.7.5 extracted from

  • Nualkaew, N.; De-Eknamkul, W.; Kutchan, T.M.; Zenk, M.H.
    Membrane-bound geranylgeranyl diphosphate phosphatases: purification and characterization from Croton stellatopilosus leaves (2006), Phytochemistry, 67, 1613-1620.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1.0 mM, complete inhibition of isoenzyme PII, 21% inhibition of isoenzyme PI Croton stellatopilosus
geranylgeranyl diphosphate substrate inhibition above 0.2 mM, isoenzyme PII. Isoenzyme PI shows no substrate inhibition Croton stellatopilosus
K+ 1.0 mM, 18% inhibition of isoenzyme PI, slight activation (1.1fold) of isoenzyme PII Croton stellatopilosus
Mn2+ 1.0 mM, complete inhibition of isoenzyme PII, 4% inhibition of isoenzyme PI Croton stellatopilosus
additional information isoenzyme PI shows no substrate inhibition by geranylgeranyl diphosphate, no inhibition by 1.0 mM Zn2+ or K+ and very low inhibition by 1 mM Na+ Croton stellatopilosus
Na+ 1.0 mM, 23% inhibition of isoenzyme PI, 5% inhibition of isoenzyme PII Croton stellatopilosus
Na2MoO4 1.0 mM, complete inhibition of isoenzyme PI and isoenzyme PII Croton stellatopilosus
Zn2+ 1.0 mM, complete inhibition of isoenzyme PII, no inhibition of isoenzyme PI Croton stellatopilosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
geranylgeranyl diphosphate pH 7.0, 30°C, isoenzyme II Croton stellatopilosus
0.2
-
geranylgeranyl diphosphate pH 7.0, 30°C, isoenzyme I Croton stellatopilosus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound to Croton stellatopilosus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 1.0 mM, 18% inhibition of isoenzyme PI, slight activation (1.1fold) of isoenzyme PII Croton stellatopilosus
additional information both activities (isoenzyme I and II) are Mg2+-independent Croton stellatopilosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30600
-
1 * 30600, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, isoenzyme PII, SDS-PAGE Croton stellatopilosus
34000
-
isoenzyme PII, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, gel filtration Croton stellatopilosus
58700
-
4 * 58700, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, isoenzyme PII, SDS-PAGE Croton stellatopilosus
232000
-
isoenzyme PI, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, gel filtration Croton stellatopilosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + H2O Croton stellatopilosus the enzyme is involved in the biosynthesis of plaunotol, a commercial anti-peptic acyclic diterpenoid geranylgeraniol + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Croton stellatopilosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of isoenzyme PI and isoenzyme PII Croton stellatopilosus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Croton stellatopilosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
isoenzyme PI Croton stellatopilosus
0.35
-
isoenzyme PII Croton stellatopilosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + H2O 11% of the activity with geranylgeranyl diphosphate, isoenzyme PI. 23% of the activity with geranylgeranyl diphosphate, isoenzyme PII Croton stellatopilosus farnesol + diphosphate
-
?
geranyl diphosphate + H2O 7% of the activity with geranylgeranyl diphosphate, isoenzyme PI. 10% of the activity with geranylgeranyl diphosphate, isoenzyme PII Croton stellatopilosus geraniol + diphosphate
-
?
geranylgeranyl diphosphate + H2O
-
Croton stellatopilosus geranylgeraniol + diphosphate
-
?
geranylgeranyl diphosphate + H2O the enzyme is involved in the biosynthesis of plaunotol, a commercial anti-peptic acyclic diterpenoid Croton stellatopilosus geranylgeraniol + diphosphate
-
?
additional information no activity with isopentenyl diphosphate, isoenzyme PI and PII Croton stellatopilosus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30600, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, isoenzyme PII, SDS-PAGE Croton stellatopilosus
tetramer 4 * 58700, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, isoenzyme PII, SDS-PAGE Croton stellatopilosus

Synonyms

Synonyms Comment Organism
GGPP phosphatase
-
Croton stellatopilosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 isoenzyme PI Croton stellatopilosus
6.5 7 isoenzyme PII Croton stellatopilosus

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 pH 5.0: about 65% of maximal activity, pH 7.0: about 60% of maximal activity, isoenzyme II Croton stellatopilosus
5 7.5 pH 5.0: about 80% of maximal activity, pH 7.0: about 45% of maximal activity, isoenzyme I Croton stellatopilosus