Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1.0 mM, complete inhibition of isoenzyme PII, 21% inhibition of isoenzyme PI | Croton stellatopilosus | |
geranylgeranyl diphosphate | substrate inhibition above 0.2 mM, isoenzyme PII. Isoenzyme PI shows no substrate inhibition | Croton stellatopilosus | |
K+ | 1.0 mM, 18% inhibition of isoenzyme PI, slight activation (1.1fold) of isoenzyme PII | Croton stellatopilosus | |
Mn2+ | 1.0 mM, complete inhibition of isoenzyme PII, 4% inhibition of isoenzyme PI | Croton stellatopilosus | |
additional information | isoenzyme PI shows no substrate inhibition by geranylgeranyl diphosphate, no inhibition by 1.0 mM Zn2+ or K+ and very low inhibition by 1 mM Na+ | Croton stellatopilosus | |
Na+ | 1.0 mM, 23% inhibition of isoenzyme PI, 5% inhibition of isoenzyme PII | Croton stellatopilosus | |
Na2MoO4 | 1.0 mM, complete inhibition of isoenzyme PI and isoenzyme PII | Croton stellatopilosus | |
Zn2+ | 1.0 mM, complete inhibition of isoenzyme PII, no inhibition of isoenzyme PI | Croton stellatopilosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
geranylgeranyl diphosphate | pH 7.0, 30°C, isoenzyme II | Croton stellatopilosus | |
0.2 | - |
geranylgeranyl diphosphate | pH 7.0, 30°C, isoenzyme I | Croton stellatopilosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Croton stellatopilosus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 1.0 mM, 18% inhibition of isoenzyme PI, slight activation (1.1fold) of isoenzyme PII | Croton stellatopilosus | |
additional information | both activities (isoenzyme I and II) are Mg2+-independent | Croton stellatopilosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30600 | - |
1 * 30600, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, isoenzyme PII, SDS-PAGE | Croton stellatopilosus |
34000 | - |
isoenzyme PII, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, gel filtration | Croton stellatopilosus |
58700 | - |
4 * 58700, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, isoenzyme PII, SDS-PAGE | Croton stellatopilosus |
232000 | - |
isoenzyme PI, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, gel filtration | Croton stellatopilosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + H2O | Croton stellatopilosus | the enzyme is involved in the biosynthesis of plaunotol, a commercial anti-peptic acyclic diterpenoid | geranylgeraniol + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Croton stellatopilosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification of isoenzyme PI and isoenzyme PII | Croton stellatopilosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Croton stellatopilosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
isoenzyme PI | Croton stellatopilosus |
0.35 | - |
isoenzyme PII | Croton stellatopilosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + H2O | 11% of the activity with geranylgeranyl diphosphate, isoenzyme PI. 23% of the activity with geranylgeranyl diphosphate, isoenzyme PII | Croton stellatopilosus | farnesol + diphosphate | - |
? | |
geranyl diphosphate + H2O | 7% of the activity with geranylgeranyl diphosphate, isoenzyme PI. 10% of the activity with geranylgeranyl diphosphate, isoenzyme PII | Croton stellatopilosus | geraniol + diphosphate | - |
? | |
geranylgeranyl diphosphate + H2O | - |
Croton stellatopilosus | geranylgeraniol + diphosphate | - |
? | |
geranylgeranyl diphosphate + H2O | the enzyme is involved in the biosynthesis of plaunotol, a commercial anti-peptic acyclic diterpenoid | Croton stellatopilosus | geranylgeraniol + diphosphate | - |
? | |
additional information | no activity with isopentenyl diphosphate, isoenzyme PI and PII | Croton stellatopilosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30600, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, isoenzyme PII, SDS-PAGE | Croton stellatopilosus |
tetramer | 4 * 58700, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, isoenzyme PII, SDS-PAGE | Croton stellatopilosus |
Synonyms | Comment | Organism |
---|---|---|
GGPP phosphatase | - |
Croton stellatopilosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | isoenzyme PI | Croton stellatopilosus |
6.5 | 7 | isoenzyme PII | Croton stellatopilosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | pH 5.0: about 65% of maximal activity, pH 7.0: about 60% of maximal activity, isoenzyme II | Croton stellatopilosus |
5 | 7.5 | pH 5.0: about 80% of maximal activity, pH 7.0: about 45% of maximal activity, isoenzyme I | Croton stellatopilosus |