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Literature summary for 3.1.7.3 extracted from
- Biosynthesis of monoterpenes: Hydrolysis of bornyl pyrophosphate, an essential step in camphor biosynthesis, and hydrolysis of geranyl pyrophosphate, the acyclic precursor of camphor, by enzymes from Sage (Salvia officinalis) (1979), Arch. Biochem. Biophys., 198, 523-532.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropylfluorophosphate | IC50: 0.8 for 65 kDa BPP hydrolase | Salvia officinalis |  |
| diphosphate | IC50: 0.6 for 65 kDa BPP hydrolase, 2.5 for 20 kDa BPP hydrolase | Salvia officinalis | |
| F- | IC50: 0.7 mM for 65 kDa BPP hydrolase, 15.5 mM for 20 kDa BPP hydrolase | Salvia officinalis | |
| Mn2+ | IC50: 0.1 mM for 65 kDa BPP hydrolase, 15.0 mM for 20 kDa BPP hydrolase | Salvia officinalis | |
| phosphate | IC50: 10.5 mM for 65 kDa BPP hydrolase, 4.3 for 20 kDa BPP hydrolase | Salvia officinalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
D-bornyl-diphosphate | 65 kDa BPP hydrolase | Salvia officinalis | |
| 0.12 | - |
D-bornyl-diphosphate | 20 kDa BPP hydrolase | Salvia officinalis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | slight stimulation at 10 mM | Salvia officinalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
gel filtration, activity on bornyl-diphosphate and bornyl-monophosphate | Salvia officinalis |
| 65000 | - |
gel filtration | Salvia officinalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-bornyl-diphosphate + H2O | Salvia officinalis | camphor biosynthesis | ? | - |
? | |
| geranyl-diphosphate + H2O | Salvia officinalis | acyclic precursor of camphor | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salvia officinalis | - |
sage | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| first step: gel filtration, second step: hydroxylapatite chromatography | Salvia officinalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Salvia officinalis | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 100 mM Tris-maleate pH 7.0, one week, 50% loss of activity for 20 kDa BPP hydrolase | Salvia officinalis |
| 0°C, 100 mM Tris-maleate pH 7.0, one week, less than 15% loss of activity for 65 kDa BPP hydrolase | Salvia officinalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-bornyl-diphosphate + H2O | - |
Salvia officinalis | D-borneol + diphosphate | - |
? | |
| D-bornyl-diphosphate + H2O | camphor biosynthesis | Salvia officinalis | ? | - |
? | |
| D-bornyl-phosphate + H2O | - |
Salvia officinalis | D-borneol + phosphate | - |
? | |
| geranyl-diphosphate + H2O | preference for geranyl-ester for 65 kDa BPP hydrolase | Salvia officinalis | geraniol + diphosphate | - |
? | |
| geranyl-diphosphate + H2O | acyclic precursor of camphor | Salvia officinalis | ? | - |
? | |
| neryl-diphosphate + H2O | - |
Salvia officinalis | nerol + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pyrophosphate hydrolase | - |
Salvia officinalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
65 kDa BPP hydrolase | Salvia officinalis |
| 7.6 | - |
20 kDa BPP hydrolase | Salvia officinalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 4.5 | 65 kDa BPP hydrolase, 33% of maximum activity above pH 4.5 and below pH 2.5 | Salvia officinalis |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
IC50: 0.1 mM for 65 kDa BPP hydrolase, 15.0 mM for 20 kDa BPP hydrolase | Salvia officinalis | Mn2+ | |
| 0.6 | - |
IC50: 0.6 mM for 65 kDa BPP hydrolase | Salvia officinalis | diphosphate | |
| 0.7 | - |
IC50: 0.7 mM for 65 kDa BPP hydrolase, 15.5 mM for 20 kDa BPP hydrolase | Salvia officinalis | F- | |
| 0.8 | - |
IC50: 0.8 mM for 65 kDa BPP hydrolase | Salvia officinalis | diisopropylfluorophosphate | |
| 2.5 | - |
IC50 2.5 mM for 20 kDa BPP hydrolase | Salvia officinalis | diphosphate | |
| 10.5 | - |
IC50: 10.5 mM for 65 kDa BPP hydrolase, 4.3 for 20 kDa BPP hydrolase | Salvia officinalis | phosphate | |
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