Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Cinnamomum tenuipile |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0558 | - |
geranyl diphosphate | pH 7.0, 30°C | Cinnamomum tenuipile |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, optimal at 10 mM | Cinnamomum tenuipile | |
Mn2+ | dependent on, optimal at 0.5 mM | Cinnamomum tenuipile | |
additional information | no effect on enzyme activity by K+ | Cinnamomum tenuipile |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
x * 69000, sequence calculation | Cinnamomum tenuipile |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + H2O | Cinnamomum tenuipile | - |
geraniol + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cinnamomum tenuipile | Q8GUE4 | gene CtGES | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Cinnamomum tenuipile |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | CtGES mRNA is localized in the oil cells of the leaves | Cinnamomum tenuipile | - |
plant oil gland | CtGES mRNA is localized in the oil cells of the leaves | Cinnamomum tenuipile | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + H2O | - |
Cinnamomum tenuipile | geraniol + diphosphate | - |
? | |
geranyl diphosphate + H2O | the monoterpene synthase specifically converts geranyl diphosphate to geraniol, GC-MS analysis | Cinnamomum tenuipile | geraniol + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 69000, sequence calculation | Cinnamomum tenuipile |
Synonyms | Comment | Organism |
---|---|---|
geraniol-synthase | - |
Cinnamomum tenuipile |
monoterpene synthase | - |
Cinnamomum tenuipile |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cinnamomum tenuipile |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Cinnamomum tenuipile |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.2 | 20% of maximal activity at pH 6.2 and pH 8.2 | Cinnamomum tenuipile |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cinnamomum tenuipile | sequence calculation | - |
5.67 |